DNA sequences and plasmids for the preparation of plants with changed sucrose concentration

ABSTRACT

PCT No. PCT/EP93/01605 Sec. 371 Date Dec. 20, 1994 Sec. 102(e) Date Dec. 20, 1994 PCT Filed Jun. 22, 1993 PCT Pub. No. WO94/00563 PCT Pub. Date Jan. 6, 1994DNA sequences are described, that by integration in a plant genome cause the activity of the sucrose-phosphate-synthase (SPS) of the plant to be changed, plasmids, containing these DNA sequences as well as transgenic plants that by introduction of the DNA sequences causes changes in the activity of sucrose-phosphate-synthase.

FIELD OF THE INVENTION

The present invention relates to DNA sequences and plasmids, containing these DNA sequences, which by integration into a plant genome, cause the activity of the sucrose-phosphate-synthase (SPS) of the plant to be changed and thus affect the sugar metabolism of the plant. The invention further relates to transgenic plants, in which through introduction of the DNA sequences, changes in the activity of the sucrose-phosphate-synthase are produced.

BACKGROUND OF THE INVENTION

Sucrose is of central importance for the plant and serves many functions. For the long distance transport of photoassimilates and/or energy between various organs in plants, sucrose is almost exclusively used. The sucrose which is transported in a specific heterotrophic organ determines the growth and the development of this organ. Thus it is known, e.g. from EP 442 592, that transgenic plants, in which the transport of the sucrose away from the exporting leaves is inhibited by expression of an apoplastic invertase, shows a strong reduction in the growth of e.g. roots or tubers in the case of potato plants. For tobacco plants, the principal importance of sucrose for the long distance transport of energy carriers within the plant is described in von Schaewen et al, 1990, EMBO J 9: 3033-3044.

While it has been clearly shown that a reduction of the amount of sucrose imported in the heterotrophic organs, such as tubers and seeds, leads to loss of yield, it is not known whether an increase in the amount of sucrose in the photosynthetically active parts of the plant, mainly the leaves, leads to a better supply of heterotrophic organs and thus to an increase in yield.

A second central role for sucrose and/or the hexoses, glucose and fructose, which are derived from sucrose, is in the protection of plants against frost damage at low temperatures. Frost damage is one of the main limiting factors in agricultural productivity in the northern hemisphere. Temperatures below freezing lead to the formation of ice crystals. Since the growing ice crystals consist of pure water, water is abstracted from the cells as the temperature falls.

This dehydration has at least two potential damaging results:

a) all dissolved substances within a cell are strongly concentrated and the cell contracts following the loss of water. Highly concentrated salts and organic acids lead to membrane damage;

b) with rehydration from dew, the previously contracted cells reexpand. The cell membrane also expands again. The volume expansion puts a heavy mechanical load on the membrane.

It is thus clear that a freezing/dew cycle can lead to severe membrane damage of the cells and thus to damage to the plant.

It thus appears worth while to hinder the freezing of plant cells. One possible strategy is to increase the formation of osmotically active substances in the cytosol of plant cells. This should lead to a lowering of the freezing point. Osmotically active substances include sucrose and/or the two hexoses which are derived from sucrose.

The increased formation of sucrose and/or the two hexoses at low temperatures is desirable in the growing plant. Another situation can exist in the harvested parts of a plant, especially in storage. For example, in potato tubers that are stored at 4°-8° C., hexoses (glucose) accumulate. It would appear to be sensible, to see this as the answer to a lowering of the temperature ("cold-sweetening").

The accumulation of sucrose and glucose has in the case of potato tubers economically undesirable results. Increased amounts of reducing sugars, such as glucose, in potatoes which are fried when preparing crisps, chips and the like, leads to an undesirable browning due to the Maillard reaction. Such products with a dark brown color are not strength is strongly dependent on the content of starch and/or its breakdown products which are important in determining the quality characteristics of the potato.

In relation to the economic aspects, sucrose thus possesses three especially important functions:

1 as the transport form for the distant transport of photoassimilates,

2 as an osmotically active substance with the desirable activity of lowering the freezing point in intact, growing plants, and

3 in the undesirable formation of reducing sugars in stored harvested parts of a plant, e.g. the potato tubers, as a result of low temperatures.

The biosynthesis pathways for the formation of sucrose, either from the primary photosynthesis products (in the leaf) or by breakdown of starch (in the storage organs e.g. of potatoes), are known. An enzyme in sucrose metabolism is sucrose-phosphate-synthase (SPS). It forms sucrose-6-phosphate from UDP-glucose and fructose-6-phosphate, which in a second step is converted to sucrose.

The isolation of SPS from maize and the cloning of a cDNA from mRNA from maize tissue is known (EP 466 995). In this application, processes for the purification of a protein such as by centrifuging of homogenates, differential precipitation and chromatography are described. A 300 times enrichment of SPS from plant tissue has been described by Salerno and Pontis (Planta 142: 41-48, 1978).

In view of the significance of SPS for carbohydrate metabolism, it is questionable whether plants can tolerate a reduction in SPS activity in all or in certain organs. It is especially not known whether it is possible to produce transgenic plants with a reduced SPS activity. Also the use of SPS for the modification of the functions of sucrose for lowering the freezing point in intact plants and for the formation of reducing sugars in harvested parts is not known.

For the preparation of plants with reduced SPS activity, i.e. plants with changed sucrose concentration, it is necessary to make available an SPS coding region of such plant species, for which processes are described, whereby transgenic plants can be grown in large numbers. In as much as a reduction of SPS activity can be achieved, by selection from a large amount, the possibility exists of obtaining plants with such a phenotype. Further organ specific promoters for gene expression should exist for the plant species, by which the possibility of an organ specific reduction of the SPS activity could be investigated.

A species which fulfils the stated requirements is Solanum tuberosum. The genetic modification of Solanum tuberosum by gene transfer using Agrobacteria is well described (Fraley et al., 1985, Crit Rev Plant Sci 4: 1-46). Promoters for leaf specific (Stockhaus et al., 1989, Plant Cell 1: 805-813), tuber specific (EP 375 092) and wound inducing (EP 375 091) gene expression are known.

SUMMARY OF THE INVENTION

The present invention now provides DNA sequences with which changes of SPS activity are actually and demonstrably possible and with which the sucrose concentration in the plant can be modified. It is concerned which include sequences with the coding region of sucrose-phosphate-synthase (SPS) from Solanum tuberosum.

These DNA sequences can be introduced in plasmids and thereby combined with steering elements for expression in eukaryotic cells. Such steering elements are, on the one hand, transcription promoters and on the other hand, transcription terminators.

Each plasmid comprises:

a) a suitable promoter, that ensures that the coding sequence is read off at the suitable time point and/or in a specified development stage in the transgenic plants or in specified tissues of transgenic plants,

b) at least one coding sequence, that

i) is coupled to the promoter so that RNA can be translated into protein, whereby the protein demonstrates enzymatic activity that leads to a modification of the sucrose concentration in the plant, or

ii) is coupled to the promoter so that the non-coding strand is read off, which leads to the formation of a so-called "anti-sense" RNA, which suppresses the formation of the coding protein of an endogenous gene in the plant which is involved in the sucrose biosynthesis, and

c) a non-coding termination sequence that contains the signals for the termination and polyadenylation of the transcript.

The present invention further provides plasmids which include DNA sequences which change the SPS activity in the plant.

The coding sequences named under b) include the SPS sequences with the following nucleotide sequences:

    __________________________________________________________________________     SPS 1 sequence (Seq. ID No.1 No.2):                                            CTATTCTCTC                                                                             CCCTCCTTTT                                                                             TCTCCTCTCT                                                                             TCAACCCCAA                                                                             AACTTCCCTT                                                                             TCAAAGCCTT                                                                             60                             TGCTTTCCCT                                                                             TTCTCACTTA                                                                             CCCAGATCAA                                                                             CTAAGCCAAT                                                                             TTGCTGTAGC                                                                             CTCAGAAAAC                                                                             120                            AGCATTCCCA                                                                             GATTGAAAAA                                                                             GAATCTTTTT                                                                             CAGTACCCAA                                                                             AAGTTGGGTT                                                                             TCTCATGTCC                                                                             180                            AGCAAGGATT                                                                             AGCTGCTCTA                                                                             GCTATTTCTT                                                                             TAGCCCTTAA                                                                             TTTTTGTCCA                                                                             GTTGTGTCTT                                                                             240                            CTGATTCTGC                                                                             ATTGGCATCT                                                                             GAATTTGATG                                                                             TGTTAAATGA                                                                             AGGGCCACCA                                                                             AAGGACTCAT                                                                             300                            ATGTAGTTGA                                                                             TGATGCTGGT                                                                             GTGCTTAGCA                                                                             GGGTGACAAA                                                                             GTCTGATTTG                                                                             AAGGCATTGT                                                                             360                            TGTCTGATGT                                                                             GGAGAAGAGA                                                                             AAAGGCTTCC                                                                             ACATTAATTT                                                                             CATCACTGTC                                                                             CGCAAGCTCA                                                                             420                            CTAGCAAAGC                                                                             TGATGCTTTT                                                                             GAGTATGCTG                                                                             ACCAAGTTTT                                                                             GGAGAAGTGG                                                                             TACCCTAGTG                                                                             480                            TTGAACAAGG                                                                             AAATGATAAG                                                                             GGTATAGTTG                                                                             TGCTTGTTAC                                                                             AAGTCAAAAG                                                                             GAAGGCGCAA                                                                             540                            TAACCGGTGG                                                                             CCCTGATTTT                                                                             GTAAAGGCCG                                                                             TTGGAGATAC                                                                             TGTTCTTGAT                                                                             GCTACCGTCT                                                                             600                            CAGAGAACCT                                                                             TCCAGTGTTG                                                                             GCTACTGAAG                                                                             AGAAGTACAA                                                                             TGAAGCAGTT                                                                             TTCAGCACTG                                                                             660                            CCACACGTCT                                                                             TGTTGCAGCC                                                                             ATTGATGGCC                                                                             TTCCTGATCC                                                                             TGGTGGACCC                                                                             CAACTCAAGG                                                                             720                            ATAACAAAAG                                                                             AGAGTCCAAC                                                                             TTCAAATCCA                                                                             GAGAGGAAAC                                                                             TGATGAGAAA                                                                             AGAGGACAAT                                                                             780                            TCACACTTGT                                                                             GGTTGGTGGG                                                                             CTGTTAGTGA                                                                             TTGCTTTTGT                                                                             TGTTCCTATG                                                                             GCTCAATACT                                                                             840                            ATGCATATGT                                                                             TTCAAAGAAG                                                                             TGAACTGTCT                                                                             GATTCTGGAA                                                                             AGTTACATTT                                                                             TCGTGAGATT                                                                             900                            TGAGTAAGCA                                                                             TGTATATTAT                                                                             CGTGTACAAA                                                                             ATGGTCCATT                                                                             CGGAAATGAC                                                                             TGATTC  956                            ATG AGA                                                                               TAT                                                                               TTA                                                                               AAA                                                                               AGG                                                                               ATA                                                                               AAT ATG                                                                               AAG                                                                               ATT                                                                               TGG                                                                               ACC                                                                               TCC                                                                               CCT 1001                           Met Arg                                                                               Tyr                                                                               Leu                                                                               Lys                                                                               Arg                                                                               Ile                                                                               Asn Met                                                                               Lys                                                                               Ile                                                                               Trp                                                                               Thr                                                                               Ser                                                                               Pro                                1            5               10             15                                 AAC ATA                                                                               ACG                                                                               GAT                                                                               ACT                                                                               GCC                                                                               ATT                                                                               TCT TTT                                                                               TCA                                                                               GAG                                                                               ATG                                                                               CTG                                                                               ACG                                                                               CCA 1046                           Asn Ile                                                                               Thr                                                                               Asp                                                                               Thr                                                                               Ala                                                                               Ile                                                                               Ser Phe                                                                               Ser                                                                               Glu                                                                               Met                                                                               Leu                                                                               Thr                                                                               Pro                                             20              25             30                                 ATA AGT                                                                               ACA                                                                               GAC                                                                               GGC                                                                               TTG                                                                               ATG                                                                               ACT GAG                                                                               ATG                                                                               GGG                                                                               GAG                                                                               AGT                                                                               AGT                                                                               GGT 1091                           Ile Ser                                                                               Thr                                                                               Asp                                                                               Gly                                                                               Leu                                                                               Met                                                                               Thr Glu                                                                               Met                                                                               Gly                                                                               Glu                                                                               Ser                                                                               Ser                                                                               Gly                                             35              40             45                                 GCT TAT                                                                               ATT                                                                               ATT                                                                               CGC                                                                               ATT                                                                               CCT                                                                               TTT GGA                                                                               CCA                                                                               AGA                                                                               GAG                                                                               AAA                                                                               TAT                                                                               ATT 1136                           Ala Tyr                                                                               Ile                                                                               Ile                                                                               Arg                                                                               Ile                                                                               Pro                                                                               Phe Gly                                                                               Pro                                                                               Arg                                                                               Glu                                                                               Lys                                                                               Tyr                                                                               Ile                                             50              55             60                                 CCA AAA                                                                               GAA                                                                               CAG                                                                               CTA                                                                               TGG                                                                               CCC                                                                               TAT ATT                                                                               CCC                                                                               GAA                                                                               TTT                                                                               GTT                                                                               GAT                                                                               GGT 1181                           Pro Lys                                                                               Glu                                                                               Gln                                                                               Leu                                                                               Trp                                                                               Pro                                                                               Tyr Ile                                                                               Pro                                                                               Glu                                                                               Phe                                                                               Val                                                                               Asp                                                                               Gly                                             65              70             75                                 GCA CTT                                                                               AAC                                                                               CAT                                                                               ATT                                                                               ATT                                                                               CAA                                                                               ATG TCC                                                                               AAA                                                                               GTT                                                                               CTT                                                                               GGG                                                                               GAG                                                                               CAA 1226                           Ala Leu                                                                               Asn                                                                               His                                                                               Ile                                                                               Ile                                                                               Gln                                                                               Met Ser                                                                               Lys                                                                               Val                                                                               Leu                                                                               Gly                                                                               Glu                                                                               Gln                                             80              85             90                                 ATT GGT                                                                               AGT                                                                               GGC                                                                               TAT                                                                               CCT                                                                               GTG                                                                               TGG CCT                                                                               GTT                                                                               GCC                                                                               ATA                                                                               CAC                                                                               GGA                                                                               CAT 1271                           Ile Gly                                                                               Ser                                                                               Gly                                                                               Tyr                                                                               Pro                                                                               Val                                                                               Trp Pro                                                                               Val                                                                               Ala                                                                               Ile                                                                               His                                                                               Gly                                                                               His                                             95              100            105                                TAT GCT                                                                               GAT                                                                               GCT                                                                               GGC                                                                               GAC                                                                               TCA                                                                               GCT GCT                                                                               CTC                                                                               CTG                                                                               TCA                                                                               GGT                                                                               GCT                                                                               TTA 1316                           Tyr Ala                                                                               Asp                                                                               Ala                                                                               Gly                                                                               Asp                                                                               Ser                                                                               Ala Ala                                                                               Leu                                                                               Leu                                                                               Ser                                                                               Gly                                                                               Ala                                                                               Leu                                             110             115            120                                AAT GTA                                                                               CCA                                                                               ATG                                                                               CTT                                                                               TTC                                                                               ACT                                                                               GGT CAC                                                                               TCA                                                                               CTT                                                                               GGT                                                                               AGA                                                                               GAT                                                                               AAG 1361                           Asn Val                                                                               Pro                                                                               Met                                                                               Leu                                                                               Phe                                                                               Thr                                                                               Gly His                                                                               Ser                                                                               Leu                                                                               Gly                                                                               Arg                                                                               Asp                                                                               Lys                                             125             130            135                                TTG GAG                                                                               CAA                                                                               CTG                                                                               TTG                                                                               CGA                                                                               CAA                                                                               GGT CGT                                                                               TTG                                                                               TCA                                                                               AAG                                                                               GAT                                                                               GAA                                                                               ATA 1406                           Leu Glu                                                                               Gln                                                                               Leu                                                                               Leu                                                                               Arg                                                                               Gln                                                                               Gly Arg                                                                               Leu                                                                               Ser                                                                               Lys                                                                               Asp                                                                               Glu                                                                               Ile                                             140             145            150                                AAC TCA                                                                               ACC                                                                               TAC                                                                               AAG                                                                               ATA                                                                               ATG                                                                               CGG AGA                                                                               ATA                                                                               GAG                                                                               GCT                                                                               GAA                                                                               GAA                                                                               TTA 1451                           Asn Ser                                                                               Thr                                                                               Tyr                                                                               Lys                                                                               Ile                                                                               Met                                                                               Arg Arg                                                                               Ile                                                                               Glu                                                                               Ala                                                                               Glu                                                                               Glu                                                                               Leu                                             155             160            165                                ACT CTT                                                                               GAT                                                                               GCT                                                                               TCC                                                                               GAA                                                                               ATT                                                                               GTC ATC                                                                               ACT                                                                               AGT                                                                               ACA                                                                               AGA                                                                               CAG                                                                               GAG 1496                           Thr Leu                                                                               Asp                                                                               Ala                                                                               Ser                                                                               Glu                                                                               Ile                                                                               Val Ile                                                                               Thr                                                                               Ser                                                                               Thr                                                                               Arg                                                                               Gln                                                                               Glu                                             170             175            180                                ATT GAC                                                                               GAG                                                                               CAA                                                                               TGG                                                                               CGT                                                                               TTG                                                                               TAT GAT                                                                               GGG                                                                               TTT                                                                               GAT                                                                               CCA                                                                               ATA                                                                               TTA 1541                           Ile Asp                                                                               Glu                                                                               Gln                                                                               Trp                                                                               Arg                                                                               Leu                                                                               Tyr Asp                                                                               Gly                                                                               Phe                                                                               Asp                                                                               Pro                                                                               Ile                                                                               Leu                                             185             190            195                                GAG CGT                                                                               AAG                                                                               TTA                                                                               CGT                                                                               GCA                                                                               AGG                                                                               ATC AAG                                                                               CGC                                                                               AAT                                                                               GTC                                                                               AGC                                                                               TGT                                                                               TAT 1586                           Glu Arg                                                                               Lys                                                                               Leu                                                                               Arg                                                                               Ala                                                                               Arg                                                                               Ile Lys                                                                               Arg                                                                               Asn                                                                               Val                                                                               Ser                                                                               Cys                                                                               Tyr                                             200             205            210                                GGC AGG                                                                               TTT                                                                               ATG                                                                               CCT                                                                               CGT                                                                               ATG                                                                               GCT GTA                                                                               ATT                                                                               CCT                                                                               CCT                                                                               GGG                                                                               ATG                                                                               GAG 1631                           Gly Arg                                                                               Phe                                                                               Met                                                                               Pro                                                                               Arg                                                                               Met                                                                               Ala Val                                                                               Ile                                                                               Pro                                                                               Pro                                                                               Gly                                                                               Met                                                                               Glu                                             215             200            225                                TTC CAC                                                                               CAT                                                                               ATT                                                                               GTG                                                                               CCA                                                                               CAT                                                                               GAA GGT                                                                               GAC                                                                               ATG                                                                               GAT                                                                               GGA                                                                               GAA                                                                               ACA 1676                           Phe His                                                                               His                                                                               Ile                                                                               Val                                                                               Pro                                                                               His                                                                               Glu Gly                                                                               Asp                                                                               Met                                                                               Asp                                                                               Gly                                                                               Glu                                                                               Thr                                             230             235            240                                GAA GGA                                                                               AGT                                                                               GAA                                                                               GAT                                                                               GGG                                                                               AAG                                                                               ACC CCG                                                                               GAT                                                                               CCA                                                                               CCT                                                                               ATT                                                                               TGG                                                                               GCA 1721                           Glu Gly                                                                               Ser                                                                               Glu                                                                               Asp                                                                               Gly                                                                               Lys                                                                               Thr Pro                                                                               Asp                                                                               Pro                                                                               Pro                                                                               Ile                                                                               Trp                                                                               Ala                                             245             250            255                                GAG ATT                                                                               ATG                                                                               CGC                                                                               TTC                                                                               TTT                                                                               TCT                                                                               AAT CCA                                                                               AGG                                                                               AAG                                                                               CCT                                                                               ATG                                                                               ATA                                                                               CTC 1766                           Glu Ile                                                                               Met                                                                               Arg                                                                               Phe                                                                               Phe                                                                               Ser                                                                               Asn Pro                                                                               Arg                                                                               Lys                                                                               Pro                                                                               Met                                                                               Ile                                                                               Leu                                             260             265            270                                GCA CTT                                                                               GCT                                                                               AGG                                                                               CCT                                                                               GAT                                                                               CCC                                                                               AAG AAG                                                                               AAC                                                                               CTC                                                                               ACT                                                                               ACT                                                                               TTA                                                                               GTG 1811                           Ala Leu                                                                               Ala                                                                               Arg                                                                               Pro                                                                               Asp                                                                               Pro                                                                               Lys Lys                                                                               Asn                                                                               Leu                                                                               Thr                                                                               Thr                                                                               Leu                                                                               Val                                             275             280            285                                AAA GCA                                                                               TTT                                                                               GGT                                                                               GAA                                                                               TGT                                                                               CGT                                                                               CCA TTG                                                                               AGA                                                                               GAG                                                                               CTT                                                                               GCT                                                                               AAT                                                                               CTT 1856                           Lys Ala                                                                               Phe                                                                               Gly                                                                               Glu                                                                               Cys                                                                               Arg                                                                               Pro Leu                                                                               Arg                                                                               Glu                                                                               Leu                                                                               Ala                                                                               Asn                                                                               Leu                                             290             295            300                                ACT TTG                                                                               ATA                                                                               ATG                                                                               GGT                                                                               AAT                                                                               CGA                                                                               GAT AAT                                                                               ATC                                                                               GAC                                                                               GAA                                                                               ATG                                                                               TCT                                                                               AGC 1901                           Thr Leu                                                                               Ile                                                                               Met                                                                               Gly                                                                               Asn                                                                               Arg                                                                               Asp Asn                                                                               Ile                                                                               Asp                                                                               Glu                                                                               Met                                                                               Ser                                                                               Ser                                             305             310            315                                ACC AAT                                                                               TCT                                                                               GCA                                                                               CTT                                                                               CTT                                                                               CTT                                                                               TCA ATC                                                                               TTG                                                                               AAA                                                                               ATG                                                                               ATA                                                                               GAT                                                                               AAG 1946                           Thr Asn                                                                               Ser                                                                               Ala                                                                               Leu                                                                               Leu                                                                               Leu                                                                               Ser Ile                                                                               Leu                                                                               Lys                                                                               met                                                                               Ile                                                                               Asp                                                                               Lys                                             320             325            330                                TAT GAT                                                                               CTT                                                                               TAT                                                                               GGT                                                                               CAA                                                                               GTA                                                                               GCT TAT                                                                               CCT                                                                               AAA                                                                               CAC                                                                               CAC                                                                               AAG                                                                               CAG 1991                           Tyr Asp                                                                               Leu                                                                               Tyr                                                                               Gly                                                                               Gln                                                                               Val                                                                               Ala Tyr                                                                               Pro                                                                               Lys                                                                               His                                                                               His                                                                               Lys                                                                               Gln                                             335             340            345                                TCA GAT                                                                               GTT                                                                               CCT                                                                               GAT                                                                               ATC                                                                               TAC                                                                               CGT CTT                                                                               GCT                                                                               GCA                                                                               AAG                                                                               ACT                                                                               AAG                                                                               GGT 2036                           Ser Asp                                                                               Val                                                                               Pro                                                                               Asp                                                                               Ile                                                                               Tyr                                                                               Arg Leu                                                                               Ala                                                                               Ala                                                                               Lys                                                                               Thr                                                                               Lys                                                                               Gly                                             350             355            360                                GTT TTT                                                                               ATT                                                                               AAT                                                                               CCA                                                                               GCT                                                                               TTT                                                                               ATT GAG                                                                               CCT                                                                               TTT                                                                               GGA                                                                               CTG                                                                               ACT                                                                               TTG 2081                           Val Phe                                                                               Ile                                                                               Asn                                                                               Pro                                                                               Ala                                                                               Phe                                                                               Ile Glu                                                                               Pro                                                                               Phe                                                                               Gly                                                                               Leu                                                                               Thr                                                                               Leu                                             365             370            375                                ATT GAG                                                                               GCA                                                                               GCA                                                                               GCT                                                                               TAT                                                                               GGT                                                                               CTC CCA                                                                               ATG                                                                               GTA                                                                               GCC                                                                               ACA                                                                               AAA                                                                               AAT 2126                           Ile Glu                                                                               Ala                                                                               Ala                                                                               Ala                                                                               Tyr                                                                               Gly                                                                               Leu Pro                                                                               Met                                                                               Val                                                                               Ala                                                                               Thr                                                                               Lys                                                                               Asn                                             380             385            390                                GGA GGA                                                                               CCT                                                                               GTT                                                                               GAT                                                                               ATA                                                                               CAT                                                                               AGG GTT                                                                               CTT                                                                               GAC                                                                               AAT                                                                               GGT                                                                               CTC                                                                               TTA 2171                           Gly Gly                                                                               Pro                                                                               Val                                                                               Asp                                                                               Ile                                                                               His                                                                               Arg Val                                                                               Leu                                                                               Asp                                                                               Asn                                                                               Gly                                                                               Leu                                                                               Leu                                             395             400            405                                GTG GAT                                                                               CCC                                                                               CAT                                                                               GAT                                                                               CAG                                                                               CAG                                                                               GCA ATT                                                                               GCT                                                                               GAT                                                                               GCT                                                                               CTT                                                                               TTG                                                                               AAG 2216                           Val Asp                                                                               Pro                                                                               His                                                                               Asp                                                                               Gln                                                                               Gln                                                                               Ala Ile                                                                               Ala                                                                               Asp                                                                               Ala                                                                               Leu                                                                               Leu                                                                               Lys                                             410             415            420                                TTG GTT                                                                               GCT                                                                               GAT                                                                               AAG                                                                               CAA                                                                               CTG                                                                               TGG GCT                                                                               AAA                                                                               TGC                                                                               AGG                                                                               GCA                                                                               AAT                                                                               GGA 2261                           Leu Val                                                                               Ala                                                                               Asp                                                                               Lys                                                                               Gln                                                                               Leu                                                                               Trp Ala                                                                               Lys                                                                               Cys                                                                               Arg                                                                               Ala                                                                               Asn                                                                               Gly                                             425             430            435                                TTA AAA                                                                               AAT                                                                               ATC                                                                               CAC                                                                               CTT                                                                               TTC                                                                               TCA TGG                                                                               CCC                                                                               GAG                                                                               CAC                                                                               TGT                                                                               AAA                                                                               ACT 2306                           Leu Lys                                                                               Asn                                                                               Ile                                                                               His                                                                               Leu                                                                               Phe                                                                               Ser Trp                                                                               Pro                                                                               Glu                                                                               His                                                                               Cys                                                                               Lys                                                                               Thr                                             440             445            450                                TAT CTA                                                                               TCC                                                                               CGG                                                                               ATA                                                                               GCT                                                                               AGC                                                                               TGC AAA                                                                               CCA                                                                               AGG                                                                               CAA                                                                               CCA                                                                               CGC                                                                               TGG 2351                           Tyr Leu                                                                               Ser                                                                               Arg                                                                               Ile                                                                               Ala                                                                               Ser                                                                               Cys Lys                                                                               Pro                                                                               Arg                                                                               Gln                                                                               Pro                                                                               Arg                                                                               Trp                                             455             460            465                                CTG AGA                                                                               TCC                                                                               ATT                                                                               GAT                                                                               GAT                                                                               GAT                                                                               GAT GAA                                                                               AAT                                                                               TCA                                                                               GAA                                                                               ACA                                                                               GAT                                                                               TCA 2396                           Leu Arg                                                                               Ser                                                                               Ile                                                                               Asp                                                                               Asp                                                                               Asp                                                                               Asp Glu                                                                               Asn                                                                               Ser                                                                               Glu                                                                               Thr                                                                               Asp                                                                               Ser                                             470             475            480                                CCT AGT                                                                               GAT                                                                               TCC                                                                               TTG                                                                               AGA                                                                               GAT                                                                               ATT CAT                                                                               GAT                                                                               ATA                                                                               TCT                                                                               CTG                                                                               AAT                                                                               TTG 2441                           Pro Ser                                                                               Asp                                                                               Ser                                                                               Leu                                                                               Arg                                                                               Asp                                                                               Ile His                                                                               Asp                                                                               Ile                                                                               Ser                                                                               Leu                                                                               Asn                                                                               Leu                                             485             490            495                                AGA TTT                                                                               TCA                                                                               TTA                                                                               GAT                                                                               GGG                                                                               GAA                                                                               AAG AAT                                                                               GAC                                                                               AAT                                                                               AAA                                                                               GAA                                                                               AAT                                                                               GCT 2486                           Arg Phe                                                                               ser                                                                               Leu                                                                               Asp                                                                               Gly                                                                               Glu                                                                               Lys Asn                                                                               Asp                                                                               Asn                                                                               Lys                                                                               Glu                                                                               Asn                                                                               Ala                                             500             505            510                                GAT AAT                                                                               ACA                                                                               TTA                                                                               GAC                                                                               CCC                                                                               GAA                                                                               GTT CGA                                                                               AGG                                                                               AGC                                                                               AAG                                                                               TTA                                                                               GAG                                                                               AAT 2531                           Asp Asn                                                                               Thr                                                                               Leu                                                                               Asp                                                                               Pro                                                                               Glu                                                                               Val Arg                                                                               Arg                                                                               Ser                                                                               Lys                                                                               Leu                                                                               Glu                                                                               Asn                                             515             520            525                                GCT GTT                                                                               TTG                                                                               TCC                                                                               TTA                                                                               TCT                                                                               AAG                                                                               GGT GCA                                                                               CTG                                                                               AAG                                                                               AGC                                                                               ACA                                                                               TCA                                                                               AAA 2576                           Ala Val                                                                               Leu                                                                               Ser                                                                               Leu                                                                               Ser                                                                               Lys                                                                               Gly Ala                                                                               Leu                                                                               Lys                                                                               Ser                                                                               Thr                                                                               Ser                                                                               Lys                                             530             535            540                                TCT TGG                                                                               TCG                                                                               TCA                                                                               GAC                                                                               AAG                                                                               GCA                                                                               GAC CAA                                                                               AAC                                                                               CCT                                                                               GGT                                                                               GCT                                                                               GGT                                                                               AAA 2621                           Ser Trp                                                                               Ser                                                                               Ser                                                                               Asp                                                                               Lys                                                                               Ala                                                                               Asp Gln                                                                               Asn                                                                               Pro                                                                               Gly                                                                               Ala                                                                               Gly                                                                               Lys                                             545             550            555                                TTC CCA                                                                               GCG                                                                               ATT                                                                               AGG                                                                               AGG                                                                               AGG                                                                               CGA CAT                                                                               ATT                                                                               TTT                                                                               GTT                                                                               ATT                                                                               GCA                                                                               GTG 2666                           Phe Pro                                                                               Ala                                                                               Ile                                                                               Arg                                                                               Arg                                                                               Arg                                                                               Arg His                                                                               Ile                                                                               Phe                                                                               Val                                                                               Ile                                                                               Ala                                                                               Val                                             560             560            565                                GAT TGT                                                                               GAT                                                                               GCT                                                                               AGC                                                                               TCA                                                                               GGA                                                                               CTC TCT                                                                               GGA                                                                               AGT                                                                               GTG                                                                               AAA                                                                               AAG                                                                               ATA 2711                           Asp Cys                                                                               Asp                                                                               Ala                                                                               Ser                                                                               Ser                                                                               Gly                                                                               Leu Ser                                                                               Gly                                                                               Ser                                                                               Val                                                                               Lys                                                                               Lys                                                                               Ile                                             570             575            580                                TTT GAG                                                                               GCT                                                                               GTA                                                                               GAG                                                                               AAG                                                                               GAA                                                                               AGG GCA                                                                               GAG                                                                               GGT                                                                               TCC                                                                               ATT                                                                               GGA                                                                               TTT 2756                           Phe Glu                                                                               Ala                                                                               Val                                                                               Glu                                                                               Lys                                                                               Glu                                                                               Arg Ala                                                                               Glu                                                                               Gly                                                                               Ser                                                                               Ile                                                                               Gly                                                                               Phe                                             585             590            595                                ATC CTG                                                                               GCT                                                                               ACA                                                                               TCT                                                                               TTC                                                                               AAT                                                                               ATA TCA                                                                               GAA                                                                               GTA                                                                               CAG                                                                               TCT                                                                               TTC                                                                               CTG 2801                           Ile Leu                                                                               Ala                                                                               Thr                                                                               Ser                                                                               Phe                                                                               Asn                                                                               Ile Ser                                                                               Glu                                                                               Val                                                                               Gln                                                                               Ser                                                                               Phe                                                                               Leu                                             600             605            610                                CTT TCA                                                                               GAG                                                                               GGC                                                                               ATG                                                                               AAT                                                                               CCT                                                                               ACT GAT                                                                               TTT                                                                               GAT                                                                               GCT                                                                               TAC                                                                               ATA                                                                               TGC 2846                           Leu Ser                                                                               Glu                                                                               Gly                                                                               Met                                                                               Asn                                                                               Pro                                                                               Thr Asp                                                                               Phe                                                                               Asp                                                                               Ala                                                                               Tyr                                                                               Ile                                                                               Cys                                             615             620            625                                AAT AGT                                                                               GGT                                                                               GGT                                                                               GAT                                                                               CTT                                                                               TAT                                                                               TAT TCG                                                                               TCC                                                                               TTC                                                                               CAT                                                                               TCT                                                                               GAG                                                                               CAA 2891                           Asn Ser                                                                               Gly                                                                               Gly                                                                               Asp                                                                               Leu                                                                               Tyr                                                                               Tyr Ser                                                                               Ser                                                                               Phe                                                                               His                                                                               Ser                                                                               Glu                                                                               Gln                                             630             635            640                                AAT CCT                                                                               TTT                                                                               GTA                                                                               GTT                                                                               GAC                                                                               TTG                                                                               TAC TAT                                                                               CAC                                                                               TCA                                                                               CAT                                                                               ATT                                                                               GAG                                                                               TAT 2936                           Asn Pro                                                                               Phe                                                                               Val                                                                               Val                                                                               Asp                                                                               Leu                                                                               Tyr Tyr                                                                               His                                                                               Ser                                                                               His                                                                               Ile                                                                               Glu                                                                               Tyr                                             645             650            655                                CGT TGG                                                                               GGG                                                                               GGC                                                                               GAA                                                                               GGA                                                                               TTG                                                                               AGA AAG                                                                               ACT                                                                               TTG                                                                               GTG                                                                               CGT                                                                               TGG                                                                               GCC 2981                           Arg Trp                                                                               Gly                                                                               Gly                                                                               Glu                                                                               Gly                                                                               Leu                                                                               Arg Lys                                                                               Thr                                                                               Leu                                                                               Val                                                                               Arg                                                                               Trp                                                                               Ala                                             660             665            670                                GCC TCT                                                                               ATC                                                                               ATT                                                                               GAT                                                                               AAG                                                                               AAT                                                                               GGT GAA                                                                               AAT                                                                               GGA                                                                               GAT                                                                               CAC                                                                               ATT                                                                               GTT 3026                           Ala Ser                                                                               Ile                                                                               Ile                                                                               Asp                                                                               Lys                                                                               Asn                                                                               Gly Glu                                                                               Asn                                                                               Gly                                                                               Asp                                                                               His                                                                               Ile                                                                               Val                                             675             680            685                                GTT GAG                                                                               GAT                                                                               GAA                                                                               GAC                                                                               AAT                                                                               TCA                                                                               GCT GAC                                                                               TAC                                                                               TGC                                                                               TAT                                                                               ACT                                                                               TTC                                                                               AAA 3071                           Val Glu                                                                               Asp                                                                               Glu                                                                               Asp                                                                               Asn                                                                               Ser                                                                               Ala Asp                                                                               Tyr                                                                               Cys                                                                               Tyr                                                                               Thr                                                                               Phe                                                                               Lys                                             690             695            700                                GTC TGC                                                                               AAG                                                                               CCT                                                                               GGG                                                                               ACG                                                                               GTT                                                                               CCT CCA                                                                               TCT                                                                               AAA                                                                               GAG                                                                               CTT                                                                               AGA                                                                               AAA 3116                           Val Cys                                                                               Lys                                                                               Pro                                                                               Gly                                                                               Thr                                                                               Val                                                                               Pro Pro                                                                               Ser                                                                               Lys                                                                               Glu                                                                               Leu                                                                               Arg                                                                               Lys                                             705             710            715                                GTA ATG                                                                               CGA                                                                               ATT                                                                               CAG                                                                               GCA                                                                               CTT                                                                               CGT TGT                                                                               CAC                                                                               GCT                                                                               GTT                                                                               TAT                                                                               TGT                                                                               CAA 3161                           Val Met                                                                               Arg                                                                               Ile                                                                               Gln                                                                               Ala                                                                               Leu                                                                               Arg Cys                                                                               His                                                                               Ala                                                                               Val                                                                               Tyr                                                                               Cys                                                                               Gln                                             720             725            730                                AAT GGG                                                                               AGT                                                                               AGG                                                                               ATT                                                                               AAT                                                                               GTG                                                                               ATC CCT                                                                               GTA                                                                               CTG                                                                               GCA                                                                               TCT                                                                               CGG                                                                               TCC 3206                           Asn Gly                                                                               Ser                                                                               Arg                                                                               Ile                                                                               Asn                                                                               Val                                                                               Ile Pro                                                                               Val                                                                               Leu                                                                               Ala                                                                               Ser                                                                               Arg                                                                               Ser                                             735             740            745                                CAA GCA                                                                               CTC                                                                               AGG                                                                               TAC                                                                               TTA                                                                               TAT                                                                               CTG CGA                                                                               TGG                                                                               GGA                                                                               ATG                                                                               GAC                                                                               TTG                                                                               TCG 3251                           Gln Ala                                                                               Leu                                                                               Arg                                                                               Tyr                                                                               Leu                                                                               Tyr                                                                               Leu Arg                                                                               Trp                                                                               Gly                                                                               Met                                                                               Asp                                                                               Leu                                                                               Ser                                             750             755            760                                AAG TTG                                                                               GTG                                                                               GTT                                                                               TTC                                                                               GTC                                                                               GGA                                                                               GAA AGT                                                                               GGT                                                                               GAT                                                                               ACC                                                                               GAT                                                                               TAT                                                                               GAA 3296                           Lys Leu                                                                               Val                                                                               Val                                                                               Phe                                                                               Val                                                                               Gly                                                                               Glu Ser                                                                               Gly                                                                               Asp                                                                               Thr                                                                               Asp                                                                               Tyr                                                                               Glu                                             765             770            775                                GGA TTA                                                                               ATC                                                                               GGT                                                                               GGT                                                                               CTA                                                                               CGC                                                                               AAG GCT                                                                               GTC                                                                               ATA                                                                               ATG                                                                               AAA                                                                               GGC                                                                               CTC 3341                           Gly Leu                                                                               Ile                                                                               Gly                                                                               Gly                                                                               Leu                                                                               Arg                                                                               Lys Ala                                                                               Val                                                                               Ile                                                                               Met                                                                               Lys                                                                               Gly                                                                               Leu                                             780             785            790                                TGC ACT                                                                               AAT                                                                               GCA                                                                               AGC                                                                               AGC                                                                               TTA                                                                               ATT CAC                                                                               GGT                                                                               AAT                                                                               AGG                                                                               AAT                                                                               TAC                                                                               CCG 3386                           Cys Thr                                                                               Asn                                                                               Ala                                                                               Ser                                                                               Ser                                                                               Leu                                                                               Ile His                                                                               Gly                                                                               Asn                                                                               Arg                                                                               Asn                                                                               Tyr                                                                               Pro                                             795             800            805                                CTA TCT                                                                               GAT                                                                               GTT                                                                               TTA                                                                               CCA                                                                               TTC                                                                               GAC AGC                                                                               CCT                                                                               AAT                                                                               GTC                                                                               ATC                                                                               CAA                                                                               GCG 3431                           Leu Ser                                                                               Asp                                                                               Val                                                                               Leu                                                                               Pro                                                                               Phe                                                                               Asp Ser                                                                               Pro                                                                               Asn                                                                               Val                                                                               Ile                                                                               Gln                                                                               Ala                                             810             815            820                                GAC GAG                                                                               GAA                                                                               TGT                                                                               AGC                                                                               AGC                                                                               ACC                                                                               GAA ATC                                                                               CGT                                                                               TGC                                                                               TTA                                                                               CTG                                                                               GTG                                                                               AAA 3476                           Asp Glu                                                                               Glu                                                                               Cys                                                                               Ser                                                                               Ser                                                                               Thr                                                                               Glu Ile                                                                               Arg                                                                               Cys                                                                               Leu                                                                               Leu                                                                               Val                                                                               Lys                                             825             830            835                                CTA GCG                                                                               GTA                                                                               CTC                                                                               AAA                                                                               GGA                                                                               TAATACCCTT                                                                             CCCCCTTTGA                                                                             TTGTCAAAAA   3524                           Leu Ala                                                                               Val                                                                               Leu                                                                               Lys                                                                               Gly                                                                         840                                                               CCTATATGAG                                                                             CTATAAGACT                                                                             ATGCCATGAA                                                                             AAGAATGGCC                                                                             ATCCATTTGG                                                                             CTTGTCTTTT                                                                             2584                           GAAGCTGTTA                                                                             ATACTTTTCA                                                                             ACAGACTACA                                                                             AAATGAGATG                                                                             AGTCCTTTGA                                                                             TCCTCTTTAA                                                                             3644                           AGGACATAAA                                                                             AGCTTTATGC                                                                             AAGAACCAGT                                                                             GCTGTAAAGT                                                                             TATAGAATTT                                                                             CTTTTGCTAT                                                                             3704                           ATATGACATT                                                                             CGACAGAACC                                                                             TGTTCCGGTT                                                                             CATCGA                  3740                           SPS 2 sequence (Seq. ID No.3 and No.4)                                         ATTTTTTTCT                                                                             CTAAGTTCTC                                                                             TCTCGCTGTC                                                                             CTTATCATTT                                                                             CACCACCTCC                                                                             ATAAATCTAG                                                                             60                             AAACATCTTT                                                                             TCTACTCCGT                                                                             TAATCTCTCT                                                                             AGCACACGGC                                                                             GGAGGAGTGC                                                                             GGCGGAGGAG                                                                             120                            ATG GCG                                                                               GGA                                                                               AAC                                                                               GAT                                                                               TGG                                                                               ATT                                                                               AAC AGT                                                                               TAC                                                                               TTA                                                                               GAG                                                                               GCG                                                                               ATA                                                                               CTG 165                            Met Ala                                                                               Gly                                                                               Asn                                                                               Asp                                                                               Trp                                                                               Ile                                                                               Asn Ser                                                                               Tyr                                                                               Leu                                                                               Glu                                                                               Ala                                                                               Ile                                                                               Leu                                1            5               10             15                                 GAT GTT                                                                               GGA                                                                               CCA                                                                               GGG                                                                               CTA                                                                               GAT                                                                               GAT AAG                                                                               AAG                                                                               TCA                                                                               TCG                                                                               TTG                                                                               TTG                                                                               TTG 210                            Asp Val                                                                               Gly                                                                               Pro                                                                               Gly                                                                               Leu                                                                               Asp                                                                               Asp Lys                                                                               Lys                                                                               Ser                                                                               Ser                                                                               Leu                                                                               Leu                                                                               Leu                                             20              25             30                                 AGA GAA                                                                               AGA                                                                               GGG                                                                               AGG                                                                               TTT                                                                               AGT                                                                               CCG ACG                                                                               AGG                                                                               TAC                                                                               TTT                                                                               GTT                                                                               GAG                                                                               GAA 255                            Arg Glu                                                                               Arg                                                                               Gly                                                                               Arg                                                                               Phe                                                                               Ser                                                                               Pro Thr                                                                               Arg                                                                               Tyr                                                                               Phe                                                                               Val                                                                               Glu                                                                               Glu                                             35              40             45                                 GTT ATT                                                                               ACT                                                                               GGA                                                                               TTC                                                                               GAT                                                                               GAG                                                                               ACT GAT                                                                               TTG                                                                               CAT                                                                               CGT                                                                               TCG                                                                               TGG                                                                               ATC 300                            Val Ile                                                                               Thr                                                                               Gly                                                                               Phe                                                                               Asp                                                                               Glu                                                                               Thr Asp                                                                               Leu                                                                               His                                                                               Arg                                                                               Ser                                                                               Trp                                                                               Ile                                             50              55             60                                 CGA GCA                                                                               CAA                                                                               GCT                                                                               ACT                                                                               CGG                                                                               AGT                                                                               CCG CAG                                                                               AGA                                                                               AGG                                                                               AAT                                                                               ACT                                                                               AGG                                                                               CTC 345                            Arg Ala                                                                               Gln                                                                               Ala                                                                               Thr                                                                               Arg                                                                               Ser                                                                               Pro Gln                                                                               Arg                                                                               Arg                                                                               Asn                                                                               Thr                                                                               Arg                                                                               Leu                                             65              70             75                                 GAG AAT                                                                               ATG                                                                               TGC                                                                               TGG                                                                               AGG                                                                               ATT                                                                               TGG AAT                                                                               TTG                                                                               GCT                                                                               CGC                                                                               CAG                                                                               AAA                                                                               AAG 390                            Glu Asn                                                                               Met                                                                               Cys                                                                               Trp                                                                               Arg                                                                               Ile                                                                               Trp Asn                                                                               Leu                                                                               Ala                                                                               Arg                                                                               Gln                                                                               Lys                                                                               Lys                                             80              85             90                                 CAG CTT                                                                               GAG                                                                               GGA                                                                               GAG                                                                               CAA                                                                               GCT                                                                               CAG TGG                                                                               ATG                                                                               GCA                                                                               AAA                                                                               CGC                                                                               CGT                                                                               CAA 435                            Gln Leu                                                                               Glu                                                                               Gly                                                                               Glu                                                                               Gln                                                                               Ala                                                                               Gln Trp                                                                               Met                                                                               Ala                                                                               Lys                                                                               Arg                                                                               Arg                                                                               Gln                                             95              100            105                                GAA CGT                                                                               GAA                                                                               AGA                                                                               GGT                                                                               CGC                                                                               AGA                                                                               GAA GCA                                                                               GTT                                                                               GCT                                                                               GAT                                                                               ATG                                                                               TCA                                                                               GAG 480                            Glu Arg                                                                               Glu                                                                               Arg                                                                               Gly                                                                               Arg                                                                               Arg                                                                               Glu Ala                                                                               Val                                                                               Ala                                                                               Asp                                                                               Met                                                                               Ser                                                                               Glu                                             110             115            120                                GAT CTA                                                                               TCT                                                                               GAG                                                                               GGA                                                                               GAG                                                                               AAA                                                                               GGA GAT                                                                               ATA                                                                               GTC                                                                               GCT                                                                               GAC                                                                               ATG                                                                               TCA 525                            Asp Leu                                                                               Ser                                                                               Glu                                                                               Gly                                                                               Glu                                                                               Lys                                                                               Gly Asp                                                                               Ile                                                                               Val                                                                               Ala                                                                               Asp                                                                               Met                                                                               Ser                                             125             130            135                                TCT CAT                                                                               GGT                                                                               GAA                                                                               AGT                                                                               ACC                                                                               AGA                                                                               GGC CGA                                                                               TTG                                                                               CCT                                                                               AGA                                                                               ATC                                                                               AGT                                                                               TCT 570                            Ser His                                                                               Gly                                                                               Glu                                                                               Ser                                                                               Thr                                                                               Arg                                                                               Gly Arg                                                                               Leu                                                                               Pro                                                                               Arg                                                                               Ile                                                                               Ser                                                                               Ser                                             140             145            150                                GTT GAG                                                                               ACA                                                                               ATG                                                                               GAA                                                                               GCA                                                                               TGG                                                                               GTC AGT                                                                               CAG                                                                               CAG                                                                               AGA                                                                               GGA                                                                               AAG                                                                               AAG 615                            Val Glu                                                                               Thr                                                                               Met                                                                               Glu                                                                               Ala                                                                               Trp                                                                               Val Ser                                                                               Gln                                                                               Gln                                                                               Arg                                                                               Gly                                                                               Lys                                                                               Lys                                             155             160            165                                CTT TAT                                                                               ATC                                                                               GTG                                                                               CTT                                                                               ATA                                                                               AGT                                                                               TTA CAT                                                                               GGT                                                                               TTA                                                                               ATT                                                                               CGG                                                                               GGT                                                                               GAG 660                            Leu Tyr                                                                               Ile                                                                               Val                                                                               Leu                                                                               Ile                                                                               Ser                                                                               Leu His                                                                               Gly                                                                               Leu                                                                               Ile                                                                               Arg                                                                               Gly                                                                               Glu                                             170             175            180                                AAT ATG                                                                               GAG                                                                               CTT                                                                               GGA                                                                               CGG                                                                               GAT                                                                               TCT GAT                                                                               ACT                                                                               GGT                                                                               GGT                                                                               CAG                                                                               GTG                                                                               AAG 705                            Asn Met                                                                               Glu                                                                               Leu                                                                               Gly                                                                               Arg                                                                               Asp                                                                               Ser Asp                                                                               Thr                                                                               Gly                                                                               Gly                                                                               Gln                                                                               Val                                                                               Lys                                             185             190            195                                TAT GTT                                                                               GTT                                                                               GAA                                                                               CTT                                                                               GCG                                                                               AGG                                                                               GCC TTA                                                                               GGG                                                                               TCG                                                                               ATG                                                                               CCA                                                                               GGT                                                                               GTA 750                            Tyr Val                                                                               Val                                                                               Glu                                                                               Leu                                                                               Ala                                                                               Arg                                                                               Ala Leu                                                                               Gly                                                                               Ser                                                                               Met                                                                               Pro                                                                               Gly                                                                               Val                                             200             205            210                                TAT CGG                                                                               GTT                                                                               GAC                                                                               TTG                                                                               CTT                                                                               ACT                                                                               AGA CAA                                                                               GTA                                                                               TCT                                                                               TCA                                                                               CCA                                                                               GAA                                                                               GTA 795                            Tyr Arg                                                                               Val                                                                               Asp                                                                               Leu                                                                               Leu                                                                               Thr                                                                               Arg Gln                                                                               Val                                                                               Ser                                                                               Ser                                                                               Pro                                                                               Glu                                                                               Val                                             215             220            225                                GAT TGG                                                                               AGC                                                                               TAT                                                                               GGT                                                                               GAG                                                                               CCG                                                                               ACA GAG                                                                               ATG                                                                               CTG                                                                               ACG                                                                               CCA                                                                               ATA                                                                               AGT 840                            Asp Trp                                                                               Ser                                                                               Tyr                                                                               Gly                                                                               Glu                                                                               Pro                                                                               Thr Glu                                                                               Met                                                                               Leu                                                                               Thr                                                                               Pro                                                                               Ile                                                                               Ser                                             230             235            240                                ACA GAC                                                                               GGC                                                                               TTG                                                                               ATG                                                                               ACT                                                                               GAG                                                                               ATG GGG                                                                               GAG                                                                               AGT                                                                               AGT                                                                               GGT                                                                               GCT                                                                               TAT 885                            Thr Asp                                                                               Gly                                                                               Leu                                                                               Met                                                                               Thr                                                                               Glu                                                                               Met Gly                                                                               Glu                                                                               Ser                                                                               Ser                                                                               Gly                                                                               Ala                                                                               Tyr                                             245             250            255                                ATT ATT                                                                               CGC                                                                               ATT                                                                               CCT                                                                               TTT                                                                               GGA                                                                               CCA AGA                                                                               GAG                                                                               AAA                                                                               TAT                                                                               ATT                                                                               CCA                                                                               AAA 930                            Ile Ile                                                                               Arg                                                                               Ile                                                                               Pro                                                                               Phe                                                                               Gly                                                                               Pro Arg                                                                               Glu                                                                               Lys                                                                               Tyr                                                                               Ile                                                                               Pro                                                                               Lys                                             260             265            270                                GAA CAG                                                                               CTA                                                                               TGG                                                                               CCC                                                                               TAT                                                                               ATT                                                                               CCC GAA                                                                               TTT                                                                               GTT                                                                               GAT                                                                               GGT                                                                               GCA                                                                               CTT 975                            Glu Gln                                                                               Leu                                                                               Trp                                                                               Pro                                                                               Tyr                                                                               Ile                                                                               Pro Glu                                                                               Phe                                                                               Val                                                                               Asp                                                                               Gly                                                                               Ala                                                                               Leu                                             275             280            285                                AAC CAT                                                                               ATT                                                                               ATT                                                                               CAA                                                                               ATG                                                                               TCC                                                                               AAA GTT                                                                               CTT                                                                               GGG                                                                               GAG                                                                               CAA                                                                               ATT                                                                               GGT 1020                           Asn His                                                                               Ile                                                                               Ile                                                                               Gln                                                                               Met                                                                               Ser                                                                               Lys Val                                                                               Leu                                                                               Gly                                                                               Glu                                                                               Gln                                                                               Ile                                                                               Gly                                             290             295            300                                AGT GGC                                                                               TAT                                                                               CCT                                                                               GTG                                                                               TGG                                                                               CCT                                                                               GTT GCC                                                                               ATA                                                                               CAC                                                                               GGA                                                                               CAT                                                                               TAT                                                                               GCT 1065                           Ser Gly                                                                               Tyr                                                                               Pro                                                                               Val                                                                               Trp                                                                               Pro                                                                               Val Ala                                                                               Ile                                                                               His                                                                               Gly                                                                               His                                                                               Tyr                                                                               Ala                                             305             310            315                                GAT GCT                                                                               GGC                                                                               GAC                                                                               TCA                                                                               GCT                                                                               GCT                                                                               CTC CTG                                                                               TCA                                                                               GGT                                                                               GCT                                                                               TTA                                                                               AAT                                                                               GTA 1110                           Asp Ala                                                                               Gly                                                                               Asp                                                                               Ser                                                                               Ala                                                                               Ala                                                                               Leu Leu                                                                               Ser                                                                               Gly                                                                               Ala                                                                               Leu                                                                               Asn                                                                               Val                                             320             330            335                                CCA ATG                                                                               CTT                                                                               TTC                                                                               ACT                                                                               GGT                                                                               CAC                                                                               TCA CTT                                                                               GGT                                                                               AGA                                                                               GAT                                                                               AAG                                                                               TTG                                                                               GAG 1155                           Pro Met                                                                               Leu                                                                               Phe                                                                               Thr                                                                               Gly                                                                               His                                                                               Ser Leu                                                                               Gly                                                                               Arg                                                                               Asp                                                                               Lys                                                                               Leu                                                                               Glu                                             340             345            350                                CAA CTG                                                                               TTG                                                                               GCA                                                                               CAA                                                                               GGT                                                                               CGA                                                                               AAG TCA                                                                               AAG                                                                               GAT                                                                               GAA                                                                               ATA                                                                               AAC                                                                               TCA 1200                           Gln Leu                                                                               Leu                                                                               Ala                                                                               Gln                                                                               Gly                                                                               Arg                                                                               Lys Ser                                                                               Lys                                                                               Asp                                                                               Glu                                                                               Ile                                                                               Asn                                                                               Ser                                             355             360            365                                ACC TAC                                                                               AAG                                                                               ATA                                                                               ATG                                                                               CGG                                                                               AGA                                                                               ATA GAG                                                                               GCT                                                                               GAA                                                                               GAA                                                                               TTA                                                                               ACT                                                                               CTT 1245                           Thr Tyr                                                                               Lys                                                                               Ile                                                                               Met                                                                               Arg                                                                               Arg                                                                               Ile Glu                                                                               Ala                                                                               Glu                                                                               Glu                                                                               Leu                                                                               Thr                                                                               Leu                                             370             375            380                                GAT GCT                                                                               TCC                                                                               GAA                                                                               ATT                                                                               GTC                                                                               ATC                                                                               ACT AGT                                                                               ACA                                                                               AGA                                                                               CAG                                                                               GAG                                                                               ATT                                                                               GAC 1290                           Asp Ala                                                                               Ser                                                                               Glu                                                                               Ile                                                                               Val                                                                               Ile                                                                               Thr Ser                                                                               Thr                                                                               Arg                                                                               Gln                                                                               Glu                                                                               Ile                                                                               Asp                                             385             390            395                                GAG CAA                                                                               TGG                                                                               CGT                                                                               TTG                                                                               TAT                                                                               GAT                                                                               GGG TTT                                                                               GAT                                                                               CCA                                                                               ATA                                                                               TTA                                                                               GAG                                                                               CGT 1335                           Glu Gln                                                                               Trp                                                                               Arg                                                                               Leu                                                                               Tyr                                                                               Asp                                                                               Gly Phe                                                                               Asp                                                                               Pro                                                                               Ile                                                                               Leu                                                                               Glu                                                                               Arg                                             400             405            410                                AAG TTA                                                                               CGT                                                                               GCA                                                                               AGG                                                                               ATC                                                                               AAG                                                                               CGC AAT                                                                               GTC                                                                               AGC                                                                               TGT                                                                               TAT                                                                               GGC                                                                               AGG 1380                           Lys Leu                                                                               Arg                                                                               Ala                                                                               Arg                                                                               Ile                                                                               Lys                                                                               Arg Asn                                                                               Val                                                                               Ser                                                                               Cys                                                                               Tyr                                                                               Gly                                                                               Arg                                             415             420            425                                TTT ATG                                                                               CCT                                                                               CGT                                                                               ATG                                                                               GCT                                                                               GTA                                                                               ATT CCT                                                                               CCT                                                                               GGG                                                                               ATG                                                                               GAG                                                                               TTC                                                                               CAC 1425                           Phe Met                                                                               Pro                                                                               Arg                                                                               Met                                                                               Ala                                                                               Val                                                                               Ile Pro                                                                               Pro                                                                               Gly                                                                               Met                                                                               Glu                                                                               Phe                                                                               His                                             430             435            440                                CAT ATT                                                                               GTG                                                                               CCA                                                                               CAT                                                                               GAA                                                                               GGT                                                                               GAC ATG                                                                               GAT                                                                               GGT                                                                               GAA                                                                               ACA                                                                               GAA                                                                               GGA 1470                           His Ile                                                                               Val                                                                               Pro                                                                               His                                                                               Glu                                                                               Gly                                                                               Asp Met                                                                               Asp                                                                               Gly                                                                               Glu                                                                               Thr                                                                               Glu                                                                               Gly                                             445             450            455                                AGT GAA                                                                               GAT                                                                               GGG                                                                               AAG                                                                               ACC                                                                               CCG                                                                               GAT CCA                                                                               CCT                                                                               ATT                                                                               TGG                                                                               GCA                                                                               GAG                                                                               ATT 1515                           Ser Glu                                                                               Asp                                                                               Gly                                                                               Lys                                                                               Thr                                                                               Pro                                                                               Asp Pro                                                                               Pro                                                                               Ile                                                                               Trp                                                                               Ala                                                                               Glu                                                                               Ile                                             460             465            470                                ATG CGC                                                                               TTC                                                                               TTT                                                                               TCT                                                                               AAT                                                                               CCA                                                                               AGG AAG                                                                               CCT                                                                               ATG                                                                               ATA                                                                               CTC                                                                               GCA                                                                               CTT 1560                           Met Arg                                                                               Phe                                                                               Phe                                                                               Ser                                                                               Asn                                                                               Pro                                                                               Arg Lys                                                                               Pro                                                                               Met                                                                               Ile                                                                               Leu                                                                               Ala                                                                               Leu                                             475             480            485                                GCT AGG                                                                               CCT                                                                               GAT                                                                               CCC                                                                               AAG                                                                               AAG                                                                               AAC CTC                                                                               ACT                                                                               ACT                                                                               TTA                                                                               GTG                                                                               AAA                                                                               GCA 1605                           Ala Arg                                                                               Pro                                                                               Asp                                                                               Pro                                                                               Lys                                                                               Lys                                                                               Asn Leu                                                                               Thr                                                                               Thr                                                                               Leu                                                                               Val                                                                               Lys                                                                               Ala                                             490             495            500                                TTT GGT                                                                               GAA                                                                               TGT                                                                               CGT                                                                               CCA                                                                               TTG                                                                               AGA GAG                                                                               CTT                                                                               GCT                                                                               AAT                                                                               CTT                                                                               ACT                                                                               TTG 1650                           Phe Gly                                                                               Glu                                                                               Cys                                                                               Arg                                                                               Pro                                                                               Leu                                                                               Arg Glu                                                                               Leu                                                                               Ala                                                                               Asn                                                                               Leu                                                                               Thr                                                                               Leu                                             505             510            515                                ATA ATG                                                                               GGT                                                                               AAT                                                                               CGA                                                                               GAT                                                                               AAT                                                                               ATC GAC                                                                               GAA                                                                               ATG                                                                               TCT                                                                               AGC                                                                               ACC                                                                               AAT 1695                           Ile Met                                                                               Gly                                                                               Asn                                                                               Arg                                                                               Asp                                                                               Asn                                                                               Ile Asp                                                                               Glu                                                                               Met                                                                               Ser                                                                               Ser                                                                               Thr                                                                               Asn                                             520             525            530                                TCT GCA                                                                               CTT                                                                               CTT                                                                               CTT                                                                               TCA                                                                               ATC                                                                               TTG AAA                                                                               ATG                                                                               ATA                                                                               GAT                                                                               AAG                                                                               TAT                                                                               GAT 1740                           Ser Ala                                                                               Leu                                                                               Leu                                                                               Leu                                                                               Ser                                                                               Ile                                                                               Leu Lys                                                                               Met                                                                               Ile                                                                               Asp                                                                               Lys                                                                               Tyr                                                                               Asp                                             535             540            540                                CTT TAT                                                                               GGT                                                                               CAA                                                                               GTA                                                                               GCT                                                                               TAT                                                                               CCT AAA                                                                               CAC                                                                               CAC                                                                               AAG                                                                               CAG                                                                               TCA                                                                               GAT 1785                           Leu Tyr                                                                               Gly                                                                               Gln                                                                               Val                                                                               Ala                                                                               Tyr                                                                               Pro Lys                                                                               His                                                                               His                                                                               Lys                                                                               Gln                                                                               Ser                                                                               Asp                                             545             550            555                                GTT CCT                                                                               GAT                                                                               ATC                                                                               TAC                                                                               CGT                                                                               CTT                                                                               GCT GCA                                                                               AAG                                                                               ACT                                                                               AAG                                                                               GGT                                                                               GTT                                                                               TTT 1830                           Val Pro                                                                               Asp                                                                               Ile                                                                               Tyr                                                                               Arg                                                                               Leu                                                                               Ala Ala                                                                               Lys                                                                               Thr                                                                               Lys                                                                               Gly                                                                               Val                                                                               Phe                                             560             565            570                                ATT AAT                                                                               CCA                                                                               GCT                                                                               TTT                                                                               ATT                                                                               GAG                                                                               CCT TTT                                                                               GGA                                                                               CTG                                                                               ACT                                                                               TTG                                                                               ATT                                                                               GAG 1875                           Ile Asn                                                                               Pro                                                                               Ala                                                                               Phe                                                                               Ile                                                                               Glu                                                                               Pro Phe                                                                               Gly                                                                               Leu                                                                               Thr                                                                               Leu                                                                               Ile                                                                               Glu                                             575             580            585                                GCA GCA                                                                               GCT                                                                               TAT                                                                               GGT                                                                               CTC                                                                               CCA                                                                               ATG GTA                                                                               GCC                                                                               ACA                                                                               AAA                                                                               AAT                                                                               GGA                                                                               GGA 1920                           Ala Ala                                                                               Ala                                                                               Tyr                                                                               Gly                                                                               Leu                                                                               Pro                                                                               Met Val                                                                               Ala                                                                               Thr                                                                               Lys                                                                               Asn                                                                               Gly                                                                               Gly                                             590             595            600                                CCT GTT                                                                               GAT                                                                               ATA                                                                               CAT                                                                               AGG                                                                               GTT                                                                               CTT GAC                                                                               AAT                                                                               GGT                                                                               CTC                                                                               TTA                                                                               GTG                                                                               GAT 1965                           Pro Val                                                                               Asp                                                                               Ile                                                                               His                                                                               Arg                                                                               Val                                                                               Leu Asp                                                                               Asn                                                                               Gly                                                                               Leu                                                                               Leu                                                                               Val                                                                               Asp                                             605             610            615                                CCC CAT                                                                               GAT                                                                               CAG                                                                               CAG                                                                               GCA                                                                               ATT                                                                               GCT GAT                                                                               GCT                                                                               CTT                                                                               TTG                                                                               AAG                                                                               TTG                                                                               GTT 2010                           Pro His                                                                               Asp                                                                               Gln                                                                               Gln                                                                               Ala                                                                               Ile                                                                               Ala Asp                                                                               Ala                                                                               Leu                                                                               Leu                                                                               Lys                                                                               Leu                                                                               Val                                             620             625            630                                GCT GAT                                                                               AAG                                                                               CAA                                                                               CTG                                                                               TGG                                                                               GCT                                                                               AAA TGC                                                                               AGG                                                                               GCA                                                                               AAT                                                                               GGA                                                                               TTA                                                                               AAA 2055                           Ala Asp                                                                               Lys                                                                               Gln                                                                               Leu                                                                               Trp                                                                               Ala                                                                               Lys Cys                                                                               Arg                                                                               Ala                                                                               Asn                                                                               Gly                                                                               Leu                                                                               Lys                                             635             640            645                                AAT ATC                                                                               CAC                                                                               CTT                                                                               TTC                                                                               TCA                                                                               TGG                                                                               CCC GAG                                                                               CAC                                                                               TGT                                                                               AAA                                                                               ACT                                                                               TAT                                                                               CTA 2100                           Asn Ile                                                                               His                                                                               Leu                                                                               Phe                                                                               Ser                                                                               Trp                                                                               Pro Glu                                                                               His                                                                               Cys                                                                               Lys                                                                               Thr                                                                               Tyr                                                                               Leu                                             650             655            660                                TCC CGG                                                                               ATA                                                                               GCT                                                                               AGC                                                                               TGC                                                                               AAA                                                                               CCA AGG                                                                               CAA                                                                               CCA                                                                               CGC                                                                               TGG                                                                               CTG                                                                               AGA 2145                           Ser Arg                                                                               Ile                                                                               Ala                                                                               Ser                                                                               Cys                                                                               Lys                                                                               Pro Arg                                                                               Gln                                                                               Pro                                                                               Arg                                                                               Trp                                                                               Leu                                                                               Arg                                             665             670            675                                TCC ATT                                                                               GAT                                                                               GAT                                                                               GAT                                                                               GAT                                                                               GAA                                                                               AAT TCA                                                                               GAA                                                                               ACA                                                                               GAT                                                                               TCA                                                                               CCT                                                                               AGT 2190                           Ser Ile                                                                               Asp                                                                               Asp                                                                               Asp                                                                               Asp                                                                               Glu                                                                               Asn Ser                                                                               Glu                                                                               Thr                                                                               Asp                                                                               Ser                                                                               Pro                                                                               Ser                                             680             685            690                                GAT TCC                                                                               TTG                                                                               AGA                                                                               GAT                                                                               ATT                                                                               CAT                                                                               GAT ATA                                                                               TCT                                                                               CTG                                                                               AAT                                                                               TTG                                                                               AGA                                                                               TTT 2235                           Asp Ser                                                                               Leu                                                                               Arg                                                                               Asp                                                                               Ile                                                                               His                                                                               Asp Ile                                                                               Ser                                                                               Leu                                                                               Asn                                                                               Leu                                                                               Arg                                                                               Phe                                             695             700            705                                TCA TTA                                                                               GAT                                                                               GGG                                                                               GAA                                                                               AAG                                                                               AAT                                                                               GAC AAT                                                                               AAA                                                                               GAA                                                                               AAT                                                                               GCT                                                                               GAT                                                                               AAT 2280                           Ser Leu                                                                               Asp                                                                               Gly                                                                               Glu                                                                               Lys                                                                               Asn                                                                               Asp Asn                                                                               Lys                                                                               Glu                                                                               Asn                                                                               Ala                                                                               Asp                                                                               Asn                                             710             715            720                                ACA TTA                                                                               GAC                                                                               CCC                                                                               GAA                                                                               GTT                                                                               CGA                                                                               AGG AGC                                                                               AAG                                                                               TTA                                                                               GAG                                                                               AAT                                                                               GCT                                                                               GTT 2325                           Thr Leu                                                                               Asp                                                                               Pro                                                                               Glu                                                                               Val                                                                               Arg                                                                               Arg Ser                                                                               Lys                                                                               Leu                                                                               Glu                                                                               Asn                                                                               Ala                                                                               Val                                             725             730            735                                TTG TCC                                                                               TTA                                                                               TCT                                                                               AAG                                                                               GGT                                                                               GCA                                                                               CTG AAG                                                                               AGC                                                                               ACA                                                                               TCA                                                                               AAA                                                                               TCT                                                                               TGG 2370                           Leu Ser                                                                               Leu                                                                               Ser                                                                               Lys                                                                               Gly                                                                               Ala                                                                               Leu Lys                                                                               Ser                                                                               Thr                                                                               Ser                                                                               Lys                                                                               Ser                                                                               Trp                                             740             745            750                                TCG TCA                                                                               GAC                                                                               AAG                                                                               GCA                                                                               GAC                                                                               CAA                                                                               AAC CCT                                                                               GGT                                                                               GCT                                                                               GGT                                                                               AAA                                                                               TTC                                                                               CCA 2415                           Ser Ser                                                                               Asp                                                                               Lys                                                                               Ala                                                                               Asp                                                                               Gln                                                                               Asn Pro                                                                               Gly                                                                               Ala                                                                               Gly                                                                               Lys                                                                               Phe                                                                               Pro                                             755             760            765                                GCG ATT                                                                               AGG                                                                               AGG                                                                               AGG                                                                               CGA                                                                               CAT                                                                               ATT TTT                                                                               GTT                                                                               ATT                                                                               GCA                                                                               GTG                                                                               GAT                                                                               TGT 2460                           Ala Ile                                                                               Arg                                                                               Arg                                                                               Arg                                                                               Arg                                                                               His                                                                               Ile Phe                                                                               Val                                                                               Ile                                                                               Ala                                                                               Val                                                                               Asp                                                                               Cys                                             770             775            780                                GAT GCT                                                                               AGC                                                                               TCA                                                                               GGA                                                                               CTC                                                                               TCT                                                                               GGA AGT                                                                               GTG                                                                               AAA                                                                               AAG                                                                               ATA                                                                               TTT                                                                               GAG 2505                           Asp Ala                                                                               Ser                                                                               Ser                                                                               Gly                                                                               Leu                                                                               Ser                                                                               Gly Ser                                                                               Val                                                                               Lys                                                                               Lys                                                                               Ile                                                                               Phe                                                                               Glu                                             785             790            795                                GCT GTA                                                                               GAG                                                                               AAG                                                                               GAA                                                                               AGG                                                                               GCA                                                                               GAG GGT                                                                               TCC                                                                               ATT                                                                               GGA                                                                               TTT                                                                               ATC                                                                               CTG 2550                           Ala Val                                                                               Glu                                                                               Lys                                                                               Glu                                                                               Arg                                                                               Ala                                                                               Glu Gly                                                                               Ser                                                                               Ile                                                                               Gly                                                                               Phe                                                                               Ile                                                                               Leu                                             800             805            810                                GCT ACA                                                                               TCT                                                                               TTC                                                                               AAT                                                                               ATA                                                                               TCA                                                                               GAA GTA                                                                               CAG                                                                               TCT                                                                               TTC                                                                               CTG                                                                               CTT                                                                               TCA 2595                           Ala Thr                                                                               Ser                                                                               Phe                                                                               Asn                                                                               Ile                                                                               Ser                                                                               Glu Val                                                                               Gln                                                                               Ser                                                                               Phe                                                                               Leu                                                                               Leu                                                                               Ser                                             815             820            825                                GAG GGC                                                                               ATG                                                                               AAT                                                                               CCT                                                                               ACT                                                                               GAT                                                                               TTT GAT                                                                               GCT                                                                               TAC                                                                               ATA                                                                               TGC                                                                               AAT                                                                               AGT 2640                           Glu Gly                                                                               Met                                                                               Asn                                                                               Pro                                                                               Thr                                                                               Asp                                                                               Phe Asp                                                                               Ala                                                                               Tyr                                                                               Ile                                                                               Cys                                                                               Asn                                                                               Ser                                             830             835            840                                GGT GGT                                                                               GAT                                                                               CTT                                                                               TAT                                                                               TAT                                                                               TCG                                                                               TCC TTC                                                                               CAT                                                                               TCT                                                                               GAG                                                                               CAA                                                                               AAT                                                                               CCT 2685                           Gly Gly                                                                               Asp                                                                               Leu                                                                               Tyr                                                                               Tyr                                                                               Ser                                                                               Ser Phe                                                                               His                                                                               Ser                                                                               Glu                                                                               Gln                                                                               Asn                                                                               Pro                                             845             850            855                                TTT GTA                                                                               GTT                                                                               GAC                                                                               TTG                                                                               TAC                                                                               TAT                                                                               CAC TCA                                                                               CAT                                                                               ATT                                                                               GAG                                                                               TAT                                                                               CGT                                                                               TGG 2730                           Phe Val                                                                               Val                                                                               Asp                                                                               Leu                                                                               Tyr                                                                               Tyr                                                                               His Ser                                                                               His                                                                               Ile                                                                               Glu                                                                               Tyr                                                                               Arg                                                                               Trp                                             860             865            870                                GGG GGC                                                                               GAA                                                                               GGA                                                                               TTG                                                                               AGA                                                                               AAG                                                                               ACT TTG                                                                               GTG                                                                               CGT                                                                               TGG                                                                               GCC                                                                               GCC                                                                               TCT 2775                           Gly Gly                                                                               Glu                                                                               Gly                                                                               Leu                                                                               Arg                                                                               Lys                                                                               Thr Leu                                                                               Val                                                                               Arg                                                                               Trp                                                                               Ala                                                                               Ala                                                                               Ser                                             875             880            885                                ATC ATT                                                                               GAT                                                                               AAG                                                                               AAT                                                                               GGT                                                                               GAA                                                                               AAT GGA                                                                               GAT                                                                               CAC                                                                               ATT                                                                               GTT                                                                               GTT                                                                               GAG 2820                           Ile Ile                                                                               Asp                                                                               Lys                                                                               Asn                                                                               Gly                                                                               Glu                                                                               Asn Gly                                                                               Asp                                                                               His                                                                               Ile                                                                               Val                                                                               Val                                                                               Glu                                             890             895            900                                GAT GAA                                                                               GAC                                                                               AAT                                                                               TCA                                                                               GCT                                                                               GAC                                                                               TAC TGC                                                                               TAT                                                                               ACT                                                                               TTC                                                                               AAA                                                                               GTC                                                                               TGC 2865                           Asp Glu                                                                               Asp                                                                               Asn                                                                               Ser                                                                               Ala                                                                               Asp                                                                               Tyr Cys                                                                               Tyr                                                                               Thr                                                                               Phe                                                                               Lys                                                                               Val                                                                               Cys                                             905             910            915                                AAG CCT                                                                               GGG                                                                               ACG                                                                               GTT                                                                               CCT                                                                               CCA                                                                               TCT AAA                                                                               GAG                                                                               CTT                                                                               AGA                                                                               AAA                                                                               GTA                                                                               ATG 2910                           Lys Pro                                                                               Gly                                                                               Thr                                                                               Val                                                                               Pro                                                                               Pro                                                                               Ser Lys                                                                               Glu                                                                               Leu                                                                               Arg                                                                               Lys                                                                               Val                                                                               Met                                             920             925            930                                CGA ATT                                                                               CAG                                                                               GCA                                                                               CTT                                                                               CGT                                                                               TGT                                                                               CAC GCT                                                                               GTT                                                                               TAT                                                                               TGT                                                                               CAA                                                                               AAT                                                                               GGG 2955                           Arg Ile                                                                               Gln                                                                               Ala                                                                               Leu                                                                               Arg                                                                               Cys                                                                               His Ala                                                                               Val                                                                               Tyr                                                                               Cys                                                                               Gln                                                                               Asn                                                                               Gly                                             935             940            945                                AGT AGG                                                                               ATT                                                                               AAT                                                                               GTG                                                                               ATC                                                                               CCT                                                                               GTA CTG                                                                               GCA                                                                               TCT                                                                               CGG                                                                               TCC                                                                               CAA                                                                               GCA 3000                           Ser Arg                                                                               Ile                                                                               Asn                                                                               Val                                                                               Ile                                                                               Pro                                                                               Val Leu                                                                               Ala                                                                               Ser                                                                               Arg                                                                               Ser                                                                               Gln                                                                               Ala                                             950             955            960                                CTC AGG                                                                               TAC                                                                               TTA                                                                               TAT                                                                               CTG                                                                               CGA                                                                               TGG GGA                                                                               ATG                                                                               GAC                                                                               TTG                                                                               TCG                                                                               AAG                                                                               TTG 3045                           Leu Arg                                                                               Tyr                                                                               Leu                                                                               Tyr                                                                               Leu                                                                               Arg                                                                               Trp Gly                                                                               met                                                                               Asp                                                                               Leu                                                                               Ser                                                                               Lys                                                                               Leu                                             965             970            975                                GTG GTT                                                                               TTC                                                                               GTC                                                                               GGA                                                                               GAA                                                                               AGT                                                                               GGT GAT                                                                               ACC                                                                               GAT                                                                               TAT                                                                               GAA                                                                               GGA                                                                               TTA 3090                           Val Val                                                                               Phe                                                                               Val                                                                               Gly                                                                               Glu                                                                               Ser                                                                               Gly Asp                                                                               Thr                                                                               Asp                                                                               Tyr                                                                               Glu                                                                               Gly                                                                               Leu                                             980             985            990                                ATC GGT                                                                               GGT                                                                               CTA                                                                               CGC                                                                               AAG                                                                               GCT                                                                               GTC ATA                                                                               ATG                                                                               AAA                                                                               GGC                                                                               CTC                                                                               TGC                                                                               ACT 3135                           Ile Gly                                                                               Gly                                                                               Leu                                                                               Arg                                                                               Lys                                                                               Ala                                                                               Val Ile                                                                               Met                                                                               Lys                                                                               Gly                                                                               Leu                                                                               Cys                                                                               Thr                                             995             1000           1005                               AAT GCA                                                                               AGC                                                                               AGC                                                                               TTA                                                                               ATT                                                                               CAC                                                                               GGT AAT                                                                               AGG                                                                               AAT                                                                               TAC                                                                               CCG                                                                               CTA                                                                               TCT 3180                           Asn Ala                                                                               Ser                                                                               Ser                                                                               Leu                                                                               Ile                                                                               His                                                                               Gly Asn                                                                               Arg                                                                               Asn                                                                               Tyr                                                                               Pro                                                                               Leu                                                                               Ser                                             1010            1015           1020                               GAT GTT                                                                               TTA                                                                               CCA                                                                               TTC                                                                               GAC                                                                               AGC                                                                               CCT AAT                                                                               GTC                                                                               ATC                                                                               CAA                                                                               GCG                                                                               GAC                                                                               GAG 3225                           Asp Val                                                                               Leu                                                                               Pro                                                                               Phe                                                                               Asp                                                                               Ser                                                                               Pro Asn                                                                               Val                                                                               Ile                                                                               Gln                                                                               Ala                                                                               Asp                                                                               Glu                                             1025            1030           1035                               GAA TGT                                                                               AGC                                                                               AGC                                                                               ACC                                                                               GAA                                                                               ATC                                                                               CGT TGC                                                                               TTA                                                                               CTG                                                                               GAG                                                                               AAA                                                                               CTA                                                                               GCG 3270                           Glu Cys                                                                               Ser                                                                               Ser                                                                               Thr                                                                               Glu                                                                               Ile                                                                               Arg Cys                                                                               Leu                                                                               Leu                                                                               Glu                                                                               Lys                                                                               Leu                                                                               Ala                                             1040            1045           1050                               GTA CTC                                                                               AAA                                                                               GGA                                                                               TAA                                                                               TACCCTTCCC                                                                             CCTTTGATTG                                                                             TCAAAAACCT      3315                           Val Leu                                                                               Lys                                                                               Gly                                                                               End                                                                         1054                                                                 ATATGAGCTA                                                                             TAAGACTATG                                                                             CCATGAAAAG                                                                             AATGGCCATC                                                                             CATTTGGCTT                                                                             GTCTTTTGAA                                                                             3375                           GCTGTTAATA                                                                             CTTTTCAACA                                                                             GACTACAAAA                                                                             TGAGATGAGT                                                                             CCTTTGATCC                                                                             TCTTTAAAGG                                                                             3435                           ACATAAAAGC                                                                             TTTATGCAAG                                                                             AACCAGTGCT                                                                             GTAAAGTTAT                                                                             AGAATTTCTT                                                                             TTGCTATATA                                                                             3495                           TGACATTCGA                                                                             CAGAACCAGT                                                                             TCCGGTTCAT                                                                             CGAGAAAAAG                                                                             AAATAAATTT                                                                             CAACTTATAA                                                                             3555                           ACATGCCTGA                                                                             TCATGTAAAT                                                                             TATCATATAC                                                                             ATCCATCGGA                                                                             AGGCATTATC                                                                             GATGGGTTAT                                                                             3615                           CAGATTTTTT                                                                             3625                                                                   SPS 3 sequence (Seq. ID No.5 and No.6)                                         ATTTTTT TCTCTAAATT                                                                             CTCTCTCACT                                                                             GTCCTTATCA                                                                             TTTCACCACC                                                                             TCCATAAATC                                                                             57                             TAGAAACATC                                                                             TTTTCTATTC                                                                             CGTTAATCTC                                                                             TCTAGCACAC                                                                             GGCGGAGTGC                                                                             GGCGGAGGAG                                                                             117                            ATG GCG                                                                               GGA                                                                               AAC                                                                               GAC                                                                               TGG                                                                               ATT                                                                               AAC AGT                                                                               TAC                                                                               TTA                                                                               GAG                                                                               GCG                                                                               ATA                                                                               CTG 162                            Met Ala                                                                               Gly                                                                               Asn                                                                               Asp                                                                               Trp                                                                               Ile                                                                               Asn Ser                                                                               Tyr                                                                               Leu                                                                               Glu                                                                               Ala                                                                               Ile                                                                               Leu                                1            5               10             15                                 GAT GTA                                                                               GGA                                                                               CCA                                                                               GGG                                                                               CTA                                                                               GAT                                                                               GAT AAG                                                                               AAA                                                                               TCA                                                                               TCG                                                                               TTG                                                                               TTG                                                                               TTG 207                            Asp Val                                                                               Gly                                                                               Pro                                                                               Gly                                                                               Leu                                                                               Asp                                                                               Asp Lys                                                                               Lys                                                                               Ser                                                                               Ser                                                                               Leu                                                                               Leu                                                                               Leu                                             20              25             30                                 AGA GAA                                                                               AGA                                                                               GGG                                                                               AGG                                                                               TTT                                                                               AGT                                                                               CCG ACG                                                                               AGG                                                                               TAC                                                                               TTT                                                                               GTT                                                                               GAG                                                                               GAA 252                            Arg Glu                                                                               Arg                                                                               Gly                                                                               Arg                                                                               Phe                                                                               Ser                                                                               Pro Thr                                                                               Arg                                                                               Tyr                                                                               Phe                                                                               Val                                                                               Glu                                                                               Glu                                             35              40             45                                 GTT ATT                                                                               ACT                                                                               GGA                                                                               TTC                                                                               GAT                                                                               GAG                                                                               ACT GAT                                                                               TTG                                                                               CAT                                                                               CGC                                                                               TCG                                                                               TGG                                                                               ATC 297                            Val Ile                                                                               Thr                                                                               Gly                                                                               Phe                                                                               Asp                                                                               Glu                                                                               Thr Asp                                                                               Leu                                                                               His                                                                               Arg                                                                               Ser                                                                               Trp                                                                               Ile                                             50              55             60                                 CGA GCA                                                                               CAA                                                                               GCT                                                                               ACT                                                                               CGG                                                                               AGT                                                                               CCG CAG                                                                               GAG                                                                               AGG                                                                               AAT                                                                               ACT                                                                               AGG                                                                               CTC 342                            Arg Ala                                                                               Gln                                                                               Ala                                                                               Thr                                                                               Arg                                                                               Ser                                                                               Pro Gln                                                                               Glu                                                                               Arg                                                                               Asn                                                                               Thr                                                                               Arg                                                                               Leu                                             65              70             75                                 GAG AAT                                                                               ATG                                                                               TGC                                                                               TGG                                                                               AGG                                                                               ATT                                                                               TGG AAT                                                                               TTG                                                                               GCT                                                                               CGC                                                                               CAG                                                                               AAA                                                                               AAG 387                            Glu Asn                                                                               Met                                                                               Cys                                                                               Trp                                                                               Arg                                                                               Ile                                                                               Trp Asn                                                                               Leu                                                                               Ala                                                                               Arg                                                                               Gln                                                                               Lys                                                                               Lys                                             80              85             90                                 CAG CTT                                                                               GAG                                                                               GGA                                                                               GAG                                                                               CAA                                                                               GCT                                                                               CAG TGG                                                                               ATG                                                                               GCA                                                                               AAA                                                                               CGC                                                                               CGT                                                                               CAA 432                            Gln Leu                                                                               Glu                                                                               Gly                                                                               Glu                                                                               Gln                                                                               Ala                                                                               Gln Trp                                                                               Met                                                                               Ala                                                                               Lys                                                                               Arg                                                                               Arg                                                                               Gln                                             95              100            105                                GAA CGT                                                                               GAG                                                                               AGA                                                                               GGT                                                                               CGC                                                                               AGA                                                                               GAA GCA                                                                               GTT                                                                               GCT                                                                               GAT                                                                               ATG                                                                               TCA                                                                               GAG 477                            Glu Arg                                                                               Glu                                                                               Arg                                                                               Gly                                                                               Arg                                                                               Arg                                                                               Glu Ala                                                                               Val                                                                               Ala                                                                               Asp                                                                               Met                                                                               Ser                                                                               Glu                                             110             115            120                                GAT CTA                                                                               TCT                                                                               GAG                                                                               GGA                                                                               GAG                                                                               AAA                                                                               GGA GAT                                                                               ATA                                                                               GTC                                                                               GCT                                                                               GAC                                                                               ATG                                                                               TCA 522                            Asp Leu                                                                               Ser                                                                               Glu                                                                               Gly                                                                               Glu                                                                               Lys                                                                               Gly Asp                                                                               Ile                                                                               Val                                                                               Ala                                                                               Asp                                                                               Met                                                                               Ser                                             125             130            135                                TCT CAT                                                                               GGT                                                                               GAA                                                                               AGT                                                                               ACC                                                                               AGA                                                                               GGC CGA                                                                               TTG                                                                               CCT                                                                               AGA                                                                               ATC                                                                               AGT                                                                               TCT 567                            Ser His                                                                               Gly                                                                               Glu                                                                               Ser                                                                               Thr                                                                               Arg                                                                               Gly Arg                                                                               Leu                                                                               Pro                                                                               Arg                                                                               Ile                                                                               Ser                                                                               Ser                                             140             145            150                                GTT GAG                                                                               ACA                                                                               ATG                                                                               GAA                                                                               GCA                                                                               TGG                                                                               GTC AGT                                                                               CAG                                                                               CAG                                                                               AGA                                                                               GGA                                                                               AAG                                                                               AAG 612                            Val Glu                                                                               Thr                                                                               Met                                                                               Glu                                                                               Ala                                                                               Trp                                                                               Val Ser                                                                               Gln                                                                               Gln                                                                               Arg                                                                               Gly                                                                               Lys                                                                               Lys                                             155             160            165                                CTT TAT                                                                               ATC                                                                               GTG                                                                               CTT                                                                               ATA                                                                               AGT                                                                               TTA CAT                                                                               GGT                                                                               TTA                                                                               ATT                                                                               CGG                                                                               GGT                                                                               GAG 657                            Leu Tyr                                                                               Ile                                                                               Val                                                                               Leu                                                                               Ile                                                                               Ser                                                                               Leu His                                                                               Gly                                                                               Leu                                                                               Ile                                                                               Arg                                                                               Gly                                                                               Glu                                             170             175            180                                AAT ATG                                                                               GAG                                                                               CTT                                                                               GGA                                                                               CGG                                                                               GAT                                                                               TCT GAT                                                                               ACT                                                                               GGT                                                                               GGT                                                                               CAG                                                                               GTG                                                                               AAG 702                            Asn Met                                                                               Glu                                                                               Leu                                                                               Gly                                                                               Arg                                                                               Asp                                                                               Ser Asp                                                                               Thr                                                                               Gly                                                                               Gly                                                                               Gln                                                                               Val                                                                               Lys                                             185             190            195                                TAT GTA                                                                               GTT                                                                               GGA                                                                               GCA                                                                               ACT                                                                               GTT                                                                               GCA CAA                                                                               GGT                                                                               CGT                                                                               TTG                                                                               TCA                                                                               AAG                                                                               GAT 747                            Tyr Val                                                                               Val                                                                               Gly                                                                               Ala                                                                               Thr                                                                               Val                                                                               Ala Gln                                                                               Gly                                                                               Arg                                                                               Leu                                                                               Ser                                                                               Lys                                                                               Asp                                             200             205            210                                GAA ATA                                                                               AAC                                                                               TCA                                                                               ACC                                                                               TAC                                                                               AAG                                                                               ATA ATG                                                                               CGG                                                                               AGA                                                                               ATA                                                                               GAG                                                                               GCT                                                                               GAA 792                            Glu Ile                                                                               Asn                                                                               Ser                                                                               Thr                                                                               Tyr                                                                               Lys                                                                               Ile Met                                                                               Arg                                                                               Arg                                                                               Ile                                                                               Glu                                                                               Ala                                                                               Glu                                             215             220            225                                GAA TTA                                                                               ACT                                                                               CTT                                                                               GAT                                                                               GCT                                                                               TCC                                                                               GAA ATT                                                                               GTC                                                                               ATC                                                                               ACT                                                                               AGT                                                                               ACA                                                                               AGA 837                            Glu Leu                                                                               Thr                                                                               Leu                                                                               Asp                                                                               Ala                                                                               Ser                                                                               Glu Ile                                                                               Val                                                                               Ile                                                                               Thr                                                                               Ser                                                                               Thr                                                                               Arg                                             230             235            240                                CAG GAG                                                                               ATT                                                                               GAC                                                                               GAG                                                                               CAA                                                                               TGG                                                                               CGT TTG                                                                               TAT                                                                               GAT                                                                               GGG                                                                               TTT                                                                               GAT                                                                               CCA 882                            Gln Glu                                                                               Ile                                                                               Asp                                                                               Glu                                                                               Gln                                                                               Trp                                                                               Arg Leu                                                                               Tyr                                                                               Asp                                                                               Gly                                                                               Phe                                                                               Asp                                                                               Pro                                             245             250            255                                ATA TTA                                                                               GAG                                                                               CGT                                                                               AAG                                                                               TTA                                                                               CGT                                                                               GCA AGG                                                                               ATC                                                                               AAG                                                                               CGC                                                                               AAT                                                                               GTC                                                                               AGC 927                            Ile Leu                                                                               Glu                                                                               Arg                                                                               Lys                                                                               Leu                                                                               Arg                                                                               Ala Arg                                                                               Ile                                                                               Lys                                                                               Arg                                                                               Asn                                                                               Val                                                                               Ser                                             260             265            270                                TGT TAT                                                                               GGC                                                                               AGG                                                                               TTT                                                                               ATG                                                                               CCT                                                                               CGT ATG                                                                               GCT                                                                               GTA                                                                               ATT                                                                               CCT                                                                               CCT                                                                               GGG 972                            Cys Tyr                                                                               Gly                                                                               Arg                                                                               Phe                                                                               Met                                                                               Pro                                                                               Arg Met                                                                               Ala                                                                               Val                                                                               Ile                                                                               Pro                                                                               Pro                                                                               Gly                                             275             280            285                                ATG GAG                                                                               TTC                                                                               CAC                                                                               CAT                                                                               ATT                                                                               GTG                                                                               CCA CAT                                                                               GAA                                                                               GGT                                                                               GAC                                                                               ATG                                                                               GAT                                                                               GGT 1017                           Met Glu                                                                               Phe                                                                               His                                                                               His                                                                               Ile                                                                               Val                                                                               Pro His                                                                               Glu                                                                               Gly                                                                               Asp                                                                               Met                                                                               Asp                                                                               Gly                                             290             295            300                                GAA ACA                                                                               GAA                                                                               GGA                                                                               AGT                                                                               GAA                                                                               GAT                                                                               GGA AAG                                                                               ACC                                                                               CCG                                                                               GAT                                                                               CCA                                                                               CCT                                                                               ATT 1062                           Glu Thr                                                                               Glu                                                                               Gly                                                                               Ser                                                                               Glu                                                                               Asp                                                                               Gly Lys                                                                               Thr                                                                               Pro                                                                               Asp                                                                               Pro                                                                               Pro                                                                               Ile                                             305             310            315                                TGG GCA                                                                               GAG                                                                               ATT                                                                               ATG                                                                               CGC                                                                               TTC                                                                               TTT TCT                                                                               AAT                                                                               CCA                                                                               AGG                                                                               AAG                                                                               CCT                                                                               ATG 1107                           Trp Ala                                                                               Glu                                                                               Ile                                                                               Met                                                                               Arg                                                                               Phe                                                                               Phe Ser                                                                               Asn                                                                               Pro                                                                               Arg                                                                               Lys                                                                               Pro                                                                               Met                                             320             330            335                                ATA CTC                                                                               GCA                                                                               CTT                                                                               GCT                                                                               AGG                                                                               CCT                                                                               GAT CCC                                                                               AAG                                                                               AAG                                                                               AAC                                                                               CTC                                                                               ACT                                                                               ACT 1152                           Ile Leu                                                                               Ala                                                                               Leu                                                                               Ala                                                                               Arg                                                                               Pro                                                                               Asp Pro                                                                               Lys                                                                               Lys                                                                               Asn                                                                               Leu                                                                               Thr                                                                               Thr                                             340             345            350                                TTA GTG                                                                               AAA                                                                               GCA                                                                               TTT                                                                               GGT                                                                               GAA                                                                               TGT CGT                                                                               CCA                                                                               TTG                                                                               AGA                                                                               GAC                                                                               CTT                                                                               GCT 1197                           Leu Val                                                                               Lys                                                                               Ala                                                                               Phe                                                                               Gly                                                                               Glu                                                                               Cys Arg                                                                               Pro                                                                               Leu                                                                               Arg                                                                               Asp                                                                               Leu                                                                               Ala                                             355             360            365                                AAT CTT                                                                               ACT                                                                               TTG                                                                               ATA                                                                               ATG                                                                               GGT                                                                               AAT CGA                                                                               GAT                                                                               AAT                                                                               ATC                                                                               GAC                                                                               GAA                                                                               ATG 1242                           Asn Leu                                                                               Thr                                                                               Leu                                                                               Ile                                                                               Met                                                                               Gly                                                                               Asn Arg                                                                               Asp                                                                               Asn                                                                               Ile                                                                               Asp                                                                               Glu                                                                               Met                                             370             375            380                                TCT AGC                                                                               ACC                                                                               AAT                                                                               TCT                                                                               GCA                                                                               CTT                                                                               CTT CTT                                                                               TCA                                                                               ATC                                                                               TTG                                                                               AAG                                                                               ATG                                                                               ATA 1287                           Ser Ser                                                                               Thr                                                                               Asn                                                                               Ser                                                                               Ala                                                                               Leu                                                                               Leu Leu                                                                               Ser                                                                               Ile                                                                               Leu                                                                               Lys                                                                               Met                                                                               Ile                                             385             390            395                                GAT AAG                                                                               TAT                                                                               GAT                                                                               CTT                                                                               TAT                                                                               GGT                                                                               CTA GTA                                                                               GCT                                                                               TAT                                                                               CCT                                                                               AAA                                                                               CAC                                                                               CAC 1332                           Asp Lys                                                                               Tyr                                                                               Asp                                                                               Leu                                                                               Tyr                                                                               Gly                                                                               Leu Val                                                                               Ala                                                                               Tyr                                                                               Pro                                                                               Lys                                                                               His                                                                               His                                             400             405            410                                AAG CAG                                                                               TCA                                                                               GAT                                                                               GTT                                                                               CCT                                                                               GAT                                                                               ATC TAC                                                                               CGT                                                                               CTT                                                                               GCT                                                                               GCA                                                                               AAG                                                                               ACT 1377                           Lys Gln                                                                               Ser                                                                               Asp                                                                               Val                                                                               Pro                                                                               Asp                                                                               Ile Tyr                                                                               Arg                                                                               Leu                                                                               Ala                                                                               Ala                                                                               Lys                                                                               Thr                                             415             420            425                                AAG GGT                                                                               GTT                                                                               TTT                                                                               ATT                                                                               AAT                                                                               CCA                                                                               GCT TTT                                                                               ATT                                                                               GAG                                                                               CCT                                                                               TTT                                                                               GGA                                                                               CTG 1422                           Lys Gly                                                                               Val                                                                               Phe                                                                               Ile                                                                               Asn                                                                               Pro                                                                               Ala Phe                                                                               Ile                                                                               Glu                                                                               Pro                                                                               Phe                                                                               Gly                                                                               Leu                                             430             435            440                                ACT TTG                                                                               ATT                                                                               GAG                                                                               GCA                                                                               GCA                                                                               GCT                                                                               TAT GGT                                                                               CTC                                                                               CCA                                                                               ATG                                                                               GTA                                                                               GCC                                                                               ACA 1467                           Thr Leu                                                                               Ile                                                                               Glu                                                                               Ala                                                                               Ala                                                                               Ala                                                                               Tyr Gly                                                                               Leu                                                                               Pro                                                                               Met                                                                               Val                                                                               Ala                                                                               Thr                                             445             450            455                                AAA AAT                                                                               GGA                                                                               GGA                                                                               CCT                                                                               GTT                                                                               GAT                                                                               ATA CAT                                                                               AGG                                                                               GTT                                                                               CTT                                                                               GAC                                                                               AAT                                                                               GGT 1512                           Lys Asn                                                                               Gly                                                                               Gly                                                                               Pro                                                                               Val                                                                               Asp                                                                               Ile His                                                                               Arg                                                                               Val                                                                               Leu                                                                               Asp                                                                               Asn                                                                               Gly                                             460             465            470                                CTC TTA                                                                               GTG                                                                               GAT                                                                               CCC                                                                               CAT                                                                               GAT                                                                               CAG CAG                                                                               GCA                                                                               ATT                                                                               GCT                                                                               GAT                                                                               GCT                                                                               CTT 1557                           Leu Leu                                                                               Val                                                                               Asp                                                                               Pro                                                                               His                                                                               Asp                                                                               Gln Gln                                                                               Ala                                                                               Ile                                                                               Ala                                                                               Asp                                                                               Ala                                                                               Leu                                             475             480            485                                TTG AAG                                                                               TTG                                                                               GTT                                                                               GCT                                                                               GAT                                                                               AAG                                                                               CAA CTG                                                                               TGG                                                                               GCT                                                                               AAA                                                                               TGC                                                                               AGG                                                                               GCA 1602                           Leu Lys                                                                               Leu                                                                               Val                                                                               Ala                                                                               Asp                                                                               Lys                                                                               Gln Leu                                                                               Trp                                                                               Ala                                                                               Lys                                                                               Cys                                                                               Arg                                                                               Ala                                             490             495            500                                AAT GGA                                                                               TTA                                                                               AAA                                                                               AAT                                                                               ATC                                                                               CAC                                                                               CTT TTC                                                                               TCA                                                                               TGG                                                                               CCC                                                                               GAG                                                                               CAC                                                                               TGT 1647                           Asn Gly                                                                               Leu                                                                               Lys                                                                               Asn                                                                               Ile                                                                               His                                                                               Leu Phe                                                                               Ser                                                                               Trp                                                                               Pro                                                                               Glu                                                                               His                                                                               Cys                                             505             510            515                                AAA ACT                                                                               TAT                                                                               CTA                                                                               TCC                                                                               CGG                                                                               ATA                                                                               GCT AGC                                                                               TGC                                                                               AAA                                                                               CCG                                                                               AGG                                                                               CAA                                                                               CAT 1692                           Lys Thr                                                                               Tyr                                                                               Leu                                                                               Ser                                                                               Arg                                                                               Ile                                                                               Ala Ser                                                                               Cys                                                                               Lys                                                                               Pro                                                                               Arg                                                                               Gln                                                                               His                                             520             525            530                                TCC TTG                                                                               AGA                                                                               GAT                                                                               ATT                                                                               CAT                                                                               GAT                                                                               ATA TCT                                                                               CTG                                                                               AAT                                                                               TTG                                                                               AGA                                                                               TTT                                                                               TCA 1737                           Ser Leu                                                                               Arg                                                                               Asp                                                                               Ile                                                                               His                                                                               Asp                                                                               Ile Ser                                                                               Leu                                                                               Asn                                                                               Leu                                                                               Arg                                                                               Phe                                                                               Ser                                             535             540            540                                TTA GAT                                                                               GGG                                                                               GAA                                                                               AAG                                                                               AAT                                                                               GAC                                                                               AAT AAA                                                                               GAA                                                                               AAT                                                                               GCT                                                                               GAT                                                                               AAT                                                                               ACA 1782                           Leu Asp                                                                               Gly                                                                               Glu                                                                               Lys                                                                               Asn                                                                               Asp                                                                               Asn Lys                                                                               Glu                                                                               Asn                                                                               Ala                                                                               Asp                                                                               Asn                                                                               Thr                                             545             550            555                                TTA GAC                                                                               CCC                                                                               GAA                                                                               GTT                                                                               CGA                                                                               AGG                                                                               AGC AAG                                                                               TTA                                                                               GAG                                                                               AAT                                                                               GCT                                                                               GTT                                                                               TTG 1827                           Leu Asp                                                                               Pro                                                                               Glu                                                                               Val                                                                               Arg                                                                               Arg                                                                               Ser Lys                                                                               Leu                                                                               Glu                                                                               Asn                                                                               Ala                                                                               Val                                                                               Leu                                             560             565            570                                TCC TTA                                                                               TCT                                                                               AAG                                                                               GGT                                                                               GCA                                                                               CTG                                                                               AAG AGC                                                                               ACA                                                                               TCA                                                                               AAA                                                                               TCT                                                                               TGG                                                                               TCG 1872                           Ser Leu                                                                               Ser                                                                               Lys                                                                               Gly                                                                               Ala                                                                               Leu                                                                               Lys Ser                                                                               Thr                                                                               Ser                                                                               Lys                                                                               Ser                                                                               Trp                                                                               Ser                                             575             580            585                                TCA GAC                                                                               AAG                                                                               GCA                                                                               GAC                                                                               CAA                                                                               AAT                                                                               CCT GGT                                                                               GCT                                                                               GGT                                                                               AAA                                                                               TTC                                                                               CCA                                                                               GCG 1917                           Ser Asp                                                                               Lys                                                                               Ala                                                                               Asp                                                                               Gln                                                                               Asn                                                                               Pro Gly                                                                               Ala                                                                               Gly                                                                               Lys                                                                               Phe                                                                               Pro                                                                               Ala                                             590             595            600                                ATT AGG                                                                               AGG                                                                               AGG                                                                               CGA                                                                               CAT                                                                               ATT                                                                               TTT GTT                                                                               ATT                                                                               GCA                                                                               GTG                                                                               GAT                                                                               TGT                                                                               GAT 1962                           Ile Arg                                                                               Arg                                                                               Arg                                                                               Arg                                                                               His                                                                               Ile                                                                               Phe Val                                                                               Ile                                                                               Ala                                                                               Val                                                                               Asp                                                                               Cys                                                                               Asp                                             605             610            615                                GCT AGC                                                                               TCA                                                                               GGA                                                                               CTC                                                                               TCT                                                                               GGA                                                                               AGT ATG                                                                               AAA                                                                               AAG                                                                               ATA                                                                               TTT                                                                               GAG                                                                               GCT 2007                           Ala Ser                                                                               Ser                                                                               Gly                                                                               Leu                                                                               Ser                                                                               Gly                                                                               Ser Met                                                                               Lys                                                                               Lys                                                                               Ile                                                                               Phe                                                                               Glu                                                                               Ala                                             620             625            630                                GTA GAG                                                                               AAG                                                                               GAA                                                                               AGG                                                                               GCA                                                                               GAG                                                                               GGT TCC                                                                               ATT                                                                               GGA                                                                               TTT                                                                               ATC                                                                               CTT                                                                               GCT 2052                           Val Glu                                                                               Lys                                                                               Glu                                                                               Arg                                                                               Ala                                                                               Glu                                                                               Gly Ser                                                                               Ile                                                                               Gly                                                                               Phe                                                                               Ile                                                                               Leu                                                                               Ala                                             635             640            645                                ACA TCT                                                                               TTC                                                                               AAT                                                                               ATA                                                                               TCA                                                                               GAA                                                                               GTA CAT                                                                               TCT                                                                               TTC                                                                               CTG                                                                               CTT                                                                               TCA                                                                               GAG 2097                           Thr Ser                                                                               Phe                                                                               Asn                                                                               Ile                                                                               Ser                                                                               Glu                                                                               Val Gln                                                                               Ser                                                                               Phe                                                                               Leu                                                                               Leu                                                                               Ser                                                                               Glu                                             650             655            660                                GGC ATG                                                                               AAT                                                                               CCT                                                                               ACT                                                                               GAG                                                                               CAA                                                                               AAT CCT                                                                               TTT                                                                               GTA                                                                               GTT                                                                               GAC                                                                               TTG                                                                               TAC 2142                           Gly Met                                                                               Asn                                                                               Pro                                                                               Thr                                                                               Glu                                                                               Gln                                                                               Asn Pro                                                                               Phe                                                                               Val                                                                               Val                                                                               Asp                                                                               Leu                                                                               Tyr                                             665             670            675                                TAT CAC                                                                               TCA                                                                               CAT                                                                               ATT                                                                               GAG                                                                               TAT                                                                               CGT TGG                                                                               GGG                                                                               GGC                                                                               GAA                                                                               GGG                                                                               TTG                                                                               AGA 2187                           Tyr His                                                                               Ser                                                                               His                                                                               Ile                                                                               Glu                                                                               Tyr                                                                               Arg Trp                                                                               Gly                                                                               Gly                                                                               Glu                                                                               Gly                                                                               Leu                                                                               Arg                                             680             685            690                                AAG ACT                                                                               TTG                                                                               GTG                                                                               CGT                                                                               TGG                                                                               GCC                                                                               GCC TCT                                                                               ATC                                                                               ATT                                                                               GAT                                                                               AAG                                                                               AAT                                                                               GGT 2232                           Lys Thr                                                                               Leu                                                                               Val                                                                               Arg                                                                               Trp                                                                               Ala                                                                               Ala Ser                                                                               Ile                                                                               Ile                                                                               Asp                                                                               Lys                                                                               Asn                                                                               Gly                                             695             700            705                                GAA AAT                                                                               GGA                                                                               GAT                                                                               CAC                                                                               ATT                                                                               GTT                                                                               GTT GAG                                                                               GAT                                                                               GAA                                                                               GAC                                                                               AAT                                                                               TCA                                                                               GCT 2277                           Glu Asn                                                                               Gly                                                                               Asp                                                                               His                                                                               Ile                                                                               Val                                                                               Val Glu                                                                               Asp                                                                               Glu                                                                               Asp                                                                               Asn                                                                               Ser                                                                               Ala                                             710             715            720                                GAC TAC                                                                               TGC                                                                               TAT                                                                               ACA                                                                               TTC                                                                               AAA                                                                               GTT TGC                                                                               AAG                                                                               CCT                                                                               GGG                                                                               ACG                                                                               GTT                                                                               CCT 2322                           Asp Tyr                                                                               Cys                                                                               Tyr                                                                               Thr                                                                               Phe                                                                               Lys                                                                               Val Cys                                                                               Lys                                                                               Pro                                                                               Gly                                                                               Thr                                                                               Val                                                                               Pro                                             725             730            735                                CCA TCT                                                                               AAA                                                                               GAA                                                                               CTT                                                                               AGA                                                                               AAA                                                                               GTA ATG                                                                               CGA                                                                               ATT                                                                               CAG                                                                               GCA                                                                               CTT                                                                               CGT 2367                           Pro Ser                                                                               Lys                                                                               Glu                                                                               Leu                                                                               Arg                                                                               Lys                                                                               Val Met                                                                               Arg                                                                               Ile                                                                               Gln                                                                               Ala                                                                               Leu                                                                               Arg                                             740             745            750                                TGT CAC                                                                               GCT                                                                               GTT                                                                               TAT                                                                               TGT                                                                               CAA                                                                               AAT GGG                                                                               AGT                                                                               AGG                                                                               ATT                                                                               AAT                                                                               GTG                                                                               ATC 2412                           Cys His                                                                               Ala                                                                               Val                                                                               Tyr                                                                               Cys                                                                               Gln                                                                               Asn Gly                                                                               Ser                                                                               Arg                                                                               Ile                                                                               Asn                                                                               Val                                                                               Ile                                             755             760            765                                CCT GTA                                                                               CTG                                                                               GCA                                                                               TCT                                                                               CGG                                                                               TCC                                                                               CAA GCA                                                                               CTC                                                                               AGG                                                                               TAC                                                                               TTA                                                                               TAT                                                                               CTG 2457                           Pro Val                                                                               Leu                                                                               Ala                                                                               Ser                                                                               Arg                                                                               Ser                                                                               Gln Ala                                                                               Leu                                                                               Arg                                                                               Tyr                                                                               Leu                                                                               Tyr                                                                               Leu                                             770             775            780                                CGA TGG                                                                               GGA                                                                               ATG                                                                               GTC                                                                               CCT                                                                               GTA                                                                               CTG GCA                                                                               TCT                                                                               CGG                                                                               TCC                                                                               CAA                                                                               GCA                                                                               CTC 2502                           Arg Trp                                                                               Gly                                                                               Met                                                                               Val                                                                               Pro                                                                               Val                                                                               Leu Ala                                                                               Ser                                                                               Arg                                                                               Ser                                                                               Gln                                                                               Ala                                                                               Leu                                             785             790            795                                AGG TAC                                                                               TTA                                                                               TAT                                                                               CTG                                                                               CGA                                                                               TGG                                                                               GGA ATG                                                                               GTC                                                                               CCT                                                                               GTA                                                                               CTG                                                                               GCA                                                                               TCT 2547                           Arg Tyr                                                                               Leu                                                                               Tyr                                                                               Leu                                                                               Arg                                                                               Trp                                                                               Gly Met                                                                               Val                                                                               Pro                                                                               Val                                                                               Leu                                                                               Ala                                                                               Ser                                             800             805            810                                CGG TCC                                                                               CAA                                                                               GCA                                                                               CTC                                                                               AGG                                                                               TAC                                                                               TTA TAT                                                                               CTG                                                                               CGA                                                                               TGG                                                                               GGA                                                                               ATG                                                                               GAC 2592                           Arg Ser                                                                               Gln                                                                               Ala                                                                               Leu                                                                               Arg                                                                               Tyr                                                                               Leu Tyr                                                                               Leu                                                                               Arg                                                                               Trp                                                                               Gly                                                                               Met                                                                               Asp                                             815             820            825                                TTG TCG                                                                               AAG                                                                               TTG                                                                               GTG                                                                               GTT                                                                               TTC                                                                               GTC GGA                                                                               GAA                                                                               AGT                                                                               GGT                                                                               GAT                                                                               ACC                                                                               GAT 2637                           Leu Ser                                                                               Lys                                                                               Leu                                                                               Val                                                                               Val                                                                               Phe                                                                               Val Gly                                                                               Glu                                                                               Ser                                                                               Gly                                                                               Asp                                                                               Thr                                                                               Asp                                             830             835            840                                TAT GAA                                                                               GGA                                                                               TTG                                                                               ATC                                                                               GGT                                                                               GGT                                                                               CTA CGC                                                                               AAG                                                                               GCT                                                                               GTC                                                                               ATA                                                                               ATG                                                                               AAA 2682                           Tyr Glu                                                                               Gly                                                                               Leu                                                                               Ile                                                                               Gly                                                                               Gly                                                                               Leu Arg                                                                               Lys                                                                               Ala                                                                               Val                                                                               Ile                                                                               Met                                                                               Lys                                             845             850            855                                GGA CTC                                                                               TGC                                                                               ACT                                                                               AAT                                                                               GCA                                                                               AGC                                                                               AGC TTA                                                                               ATT                                                                               CAC                                                                               GGT                                                                               AAT                                                                               AGG                                                                               AAT 2727                           Gly Leu                                                                               Cys                                                                               Thr                                                                               Asn                                                                               Ala                                                                               Ser                                                                               Ser Leu                                                                               Ile                                                                               His                                                                               Gly                                                                               Asn                                                                               Arg                                                                               Asn                                             860             865            870                                TAC CCG                                                                               CTA                                                                               TCT                                                                               GAT                                                                               GTT                                                                               TTA                                                                               CCA TTC                                                                               GAG                                                                               AGC                                                                               CCT                                                                               AAT                                                                               GTC                                                                               ATC 2772                           Tyr Pro                                                                               Leu                                                                               Ser                                                                               Asp                                                                               Val                                                                               Leu                                                                               Pro Phe                                                                               Glu                                                                               Ser                                                                               Pro                                                                               Asn                                                                               Val                                                                               Ile                                             875             880            885                                CAA GCG                                                                               GAT                                                                               GAG                                                                               GAA                                                                               TGT                                                                               AGC                                                                               AGC ACC                                                                               GGA                                                                               ATC                                                                               CGT                                                                               TCC                                                                               TTA                                                                               CTG 2817                           Gln Ala                                                                               Asp                                                                               Glu                                                                               Glu                                                                               Cys                                                                               Ser                                                                               Ser Thr                                                                               Glu                                                                               Ile                                                                               Arg                                                                               Ser                                                                               Leu                                                                               Leu                                             905             910            915                                GAG AAA                                                                               CTA                                                                               GCG                                                                               GTA                                                                               CTC                                                                               AAA                                                                               GGA TAA                                                                               TACCCTTCCC                                                                             CCTTTGATTG 2864                           Glu Lys                                                                               Leu                                                                               Ala                                                                               Val                                                                               Leu                                                                               Lys                                                                               Gly End                                                               920                                                               TCAAAAACCT                                                                             ATATGAGCTA                                                                             AGATTATGCC                                                                             ATGAAAAGAA                                                                             TGGCCATCCA                                                                             TTTGGCTTGT                                                                             2924                           CTTTTG  2930                                                                   __________________________________________________________________________

All sequences are cDNA sequences and stem from a CDNA library of leaf tissue. The expression gene is the same in various plant tissues. As a promoter, there can generally be used any promoter which is active in plants. The promoter should ensure that the foreign gene is expressed in the plant. The promoter can be chosen so that the expression occurs only in specified tissues, at a determined time point in the plant's development or at a time point determined by outside influences. The promoter can be homologous or heterologous to the plant. Suitable promoters are e.g. the promoter of the 35S RNA of the cauliflower mosaic virus, the patatin promoter B33 (Rocha-Sosa et al. (1989) EMBO J 8: 23-29) or a promoter that ensures expression only in photosynthetically active tissues. Other promoters can be used which ensure expression only in specified organs, such as the root, tuber, seed, stem or specified cell types such as mesophyllic, epidermal or transport cells. For hindering cold sweetening, suitable promoters are those which ensure an activation of the transcription is stored in harvested parts of the plants. For this, there can be considered cold induced promoters or such promoters that become active during the transition of the tuber from the phase where it stores material to the phase where it gives up material.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 provides the structure of the 35S-anti-pot-SPS gene;

FIG. 2 provides the structure of the B33-anti-pot-SPS gene;

FIG. 3 provides an analysis of plants transformed with a plasmid which included the 35S-anti-pot-SPS gene; and

FIG. 4 is an analysis of plants transformed with a plasmid which included the B33-anti-pot-SPS gene.

DETAILED DESCRIPTION OF THE INVENTION

The coding sequence contains the information for the formation of an mRNA for the sucrose-phosphate-synthase (SPS) or for the formation of an anti-sense RNA for the SPS. Whether the translatable mRNA or an anti-sense RNA is formed depends on the orientation of the coding sequence in relation to the promoter. If the 3' end of the coding sequence is fused to the 3' end of the promoter, an anti-sense RNA results, while the fusion of the 5' end of the coding sequence to the 3' end of the promoter, a translatable RNA results. This latter leads to an increase of the SPS activity in the cell, while the first leads to a reduction of the SPS activity in the cell. Such a reduction of SPS activity is especially significant in view of the undesirable formation of sucrose and/or reducing sugars as a result of cold storage of harvested organs.

The coding sequence for SPS can be one of the three described above or one that is derived by modifications of the sequences described above. A derivation can be carried out, e.g. by current methods of mutagenesis and/or recombination. In particular, changes of SPS sequences that lead to a neutralisation of the plant's own regulation mechanism are contemplated.

The DNA sequences of the invention can also be used for the preparation of derivatives whose gene products are not subjected to the plant's own activity regulation during a phosphorylation reaction.

Further, the sequences can also be used for the preparation of derivatives by targeted and non-targeted mutagenesis.

The invention relates further to derivatives of the DNA sequences of the invention that are obtained by exchange of single bases or by deletion or insertion of base sequences and which code for proteins with a comparable activity to sucrose-phosphate-synthase.

The 5' untranslated area of the sequence, Seq. ID No 1, is not part of SPS, but is shown as a cloning artefact. The methionine start codon of the coding region lies in a region in which there is no homology of the amino acid sequence to the other SPS sequences. Since this sequence does not also fully coincide in the homologous region with one of the other sequences, it is recognisable that the sequence Seq. ID No. 1 is not a derivative of the sequences Seq. ID No. 3 and Seq. ID No. 5.

The termination sequence provides the correct finishing of the transcription and the attachment of a polyadenyl group to the RNA. This polyadenyl group has an important function in the stabilisation of RNA molecules in the cells. With suitable plasmids which contain the DNA sequences of the invention, plants can be transformed with the object of raising and/or reducing the SPS activity and/or modifying of the sucrose concentration.

Plasmids that can be used include e.g. p35S-anti-pot-SPS (DSM 7125) and pB33-anti-pot-SPS (DSM 7124). With the gene 35S-anti-pot-SPS, located on the plasmid p35S-anti-pot-SPS, the concentration of the mRNA for the SPS protein and the enzymatic activity, for example, can be reduced. With the gene B33S-anti-pot-SPS, located on the plasmid pB33-anti-pot-SPS, the concentration of the mRNA for the SPS protein and the enzymatic activity, specifically for potato tubers for example, can be reduced. In a similar way to the SPS sequence (Seq. ID No. 1) located on this plasmid, other SPS sequences, e.g. the sequences Seq. ID No. 3 and Seq. ID No. 5 also be cloned in suitable vectors and for the same purpose.

In the plant, the SPS is subjected to an activity control by phosphorylation. This allows the plant to regulate the activity of the enzyme within a fixed frame independent of the amount of the SPS protein. If one of the changes occurring outside the activity of the SPS is to achieved, it is necessary to evade the plant's own regulation mechanism. Therefore changing the phosphorylation possibilities is an important target for influencing the SPS activity and thus the sucrose content of the plant.

It is not known in which position in the SPS protein, target directed changes of the coding regions can be achieved which serve the purpose of introducing in the plant SPS activity which is not subject to any of the plant's own controls.

The DNA sequence described here, which contains the coding region for SPS from Solanum tuberosum, allows the identification of the sites of protein phosphorylation of the SPS. By using standard methods (Sambrook, J., Fritsch, E. F., Maniatis, T. (1989) Molecular Cloning: A laboratory Manual, 2nd. Edn., Cold Spring Harbor Laboratory Press, N.Y., USA), a localisation of the phosphorylation positions of SPS is possible using the DNA sequences of the invention. These being known, by use of the plasmids with the SPS sequence, a target directed mutagenesis (Sambrook et al, 1989) of the coding region of SPS and/or a non-target directed mutagenesis (Sambrook et al, 1989) and subsequent probing of the desired mutations of the coding region of the SPS can be undertaken. Derivatives of the coding region can be prepared with the help of this plasmid, whose derived proteins are not subjected to the plants own regulation mechanisms.

Since the SPS enzyme is regulated by phosphorylation in all tested species, except maize, one can refer to sequence comparisons to identify possible phosphorylation sites. The criterium for this is that a serine residue appears in an acidic medium in the regulated SPS protein, but not however with maize.

There are 12 such serine residues in the sequences, Seq. ID No. 3 and Seq ID No. 5. In the sequence Seq ID No. 1, the first of the 12 serine residues is missing, since the coding region begins just later. The sequence, Seq. ID No. 1 is thus especially suitable for the production of SPS activity in plants that is not liable to endogenous activity regulation.

For the introduction of the SPS sequence in higher plants, a large number of cloning vectors are available which contain a replication signal for E. coli and a marker, which allows for the selection of the transformed cells. Examples of vectors are pBR 322, pUC-series, M13 mp-series, pACYC 184; EMBL 3 etc. According to the introduction method of the desired gene in the plant, other DNA sequences may be suitable. Should the Ti- or Ri-plasmid be used, e.g. for the transformation of the plant cell, then at least the right boundary, often however both the right and left boundary of the Ti- and Ri-Plasmid T-DNA, is attached, as a flanking region, to the gene being introduced. The use of T-DNA for the transformation of plants cells has been intensively researched and is well described in EP 120 516; Hoekama, In: The Binary Plant Vector System, Offset-drukkerij Kanters B. V. Alblasserdam, (1985), Chapter V; Fraley, et al., Crit. Rev. Plant Sci., 4:1-46 and An et al. (1985) EMBO J. 4: 277-287. Once the introduced DNA is integrated in the genome, it is generally stable there and remains also in the offspring of the original transformed cells. It normally contains a selection marker, which induces resistance in the transformed plant cells against a biocide or antibiotic such as kanamycin, G 418, bleomycin, hygromycin or phosphinotricin etc. The individual marker employed should therefore allow the selection of transformed cells from cells which lack the introduced DNA.

For the introduction of DNA into a plant, besides transformation using Agrobacteria, there are many other techniques available. These techniques include the fusion of protoplasts, microinjection of DNA and electroporation, as well as ballistic methods and virus infection. From the transformed plant material, whole plants can be regenerated in a suitable medium which contains antibiotics or biocides for selection. The resulting plants can then be tested for the presence of introduced DNA. No special demands are placed on the plasmids in injection and electroporation. Simple plasmids, such as e.g. pUC-derivatives can be used. Should however whole plants be regenerated from such transformed cells the presence of a selectable marker gene is necessary. The transformed cells grow within the plants in the usual manner (see also McCormick et al.(1986) Plant Cell Reports 5: 81-84). These plants can be grown normally and crossed with plants, that possess the same transformed genes or different genes. The resulting hybrid individuals have the corresponding phenotypical properties.

Deposits

The following plasmids were deposited at the Deutschen Sammlung. von Mikroorganismen (DSM) in Braunschweig, Germany on the 12.06.1992 (deposit number):

Plasmid p35S-anti-pot-SPS (DSM 7125)

Plasmid pB33-anti-pot-SPS (DSM 7124)

DESCRIPTION OF THE FIGURES

FIG. 1: Structure of the 35S-anti-pot-SPS gene

A=Fragment A: CaMV 35S promoter, nt 6909-7437 (Franck et al.,1980, Cell 21: 285-294)

B=Fragment B: sucrose phosphate synthase, EcoRV Fragment (nt 1 bis 2011), ca. 2000 bp, orientation: anti-sense

C=Fragment C: nt 11748-11939 of the T-DNA of the Ti-plasmid pTiACH5; Gielen et al., 1984, EMBO J 3: 835-846)

FIG. 2: Structure of the B33-anti-pot-SPS gene

A=Fragment A: B33 promoter of the patatin gene from S. tuberosum, (Rocha-Sosa et al., 1989, EMBO J 8: 23-29), ca 530 bp

B=Fragment B: sucrose phosphate synthase (s. FIG. 2), EcoRV fragment (nt 2011 bis 1), ca. 2000 bp, orientation: anti-sense

C=Fragment C: nt 11748-11939 of T-DNA of the Ti-plasmid pTiACH5 (Gielen et al., 1984, EMBO J 3: 835-846)

FIG. 3: shows the results of the transformation of transgenic potato plants.

Control=wild type plants

1-75=individual transgenic plants

FIG. 4: shows the results of the transformation of potato plants

Control=wild type plants

3-20=individual transgenic plants

In order to understand the examples forming the basis of this invention all the processes necessary for these tests and which are known per se will first of all be listed:

1. Cloning Process

The vectors pUC 18/19 and M13mp10 series (Yanisch-Perron et al. (1985) Gene 33: 103-119), as well as the vector EMBL 3 (Frischauf et al. (1983) J Mol Biol 170: 827-842) were used for cloning.

For the plant transformations, the gene constructs were cloned in the binary vector BIN 19 (Bevan (1984) Nucl. Acids Res 12: 8711-8720)

2. Bacterial strains

The E. coli strain BMH71-18 (Messing et al., Proc. Natl. Acad. Sci. USA (1977), 24, 6342-6346) or TB1 was used for the pUC and M13 mP vectors.

For the vector BIN19, only the E. coli strain TB1 was used. TB1 is a recombinant-negative, tetracycline-resistant derivative of strain JM101 (Yanisch-Perron et al., Gene (1985), 33, 103-119). The genotype of the TB1 strain is (Bart Barrel, personal communication): F'(traD36, proAB, lacI, lacZΔM15), Δ(lac, pro), SupE, this, recA, Sr1::Tn10(TcR).

The transformation of the plasmids into the potato plants was carried out using Agrobacterium tumefaciens strain LBA4404 (Bevan, (1984), Nucl. Acids Res. 12, 8711-8720).

3. Transformation of Aarobacterium tumefaciens

In the case of BIN19 derivatives, the insertion of the DNA into the Agrobacterium was effected by direct transformation in accordance with the method of Holsters et al., (1978) (Mol Gene Genet 163: 181-187). The plasmid DNA of the transformed Agrobacterium was isolated in accordance with the method of Birnboim and Doly (1979) (Nucl Acids Res 7: 1513-1523) and was analysed by gel electrophoresis after suitable restriction cleavage.

4. Plant transformation

Ten small leaves, wounded with a scalpel, of a sterile potato culture were placed in 10 ml of MS medium with 2% sucrose containing 30-50 μl of an Agrobacterium tumefaciens overnight culture grown under selection. After 3-5 minutes gentle shaking, the leaves were laid out on MS medium of 1.6% glucose, 2 mg/l of zeatin ribose, 0.02 mg/l of naphthylacetic acid, 0.02 mg/l of gibberellic acid, 500 mg/l of claforan, 50 mg/l of kanamycin and 0.8% bacto agar. After incubation for one week at 25° C. and 3000 lux, the claforan concentration in the medium was reduced by half.

5. SPS activity test

The SPS activity was determined according to the method of Siegel and Stitt (1990, Plant Science 66: 205-210) in a two stage analysis. To 180 μl of a solution of 50 mM HEPES/KOH (pH 7.4), 5 mM magnesium chloride, 5 mM fructose-6-phosphate, 25 mM glucose-6-phosphate and 6 mM uridine-5'-diphosphoglucose 20 μl of probe was added and incubated for 10 minutes at 25° C. It was heated for 3 minutes at 95° C., to complete the reaction. After centrifuging, the supernatant was spectroscopically analysed for the liberation of uridine-5'-diphosphate, whereby a pyruvate-kinase coupling enzyme reaction was used. Preparations without hexose phosphate, as well as the measurement of the recovery of added uridine-5'-diphosphate act as controls.

EXAMPLES Example 1

Cloning of genes of the sucrose-phosphate-synthase from potato

Poly-A+ RNA was isolated from large leaves of spinach plants as well as potato plants grown in the greenhouse. Resulting from the poly-A+ RNA, a cDNA library in the expression vector Lambda Zap II was laid out. 100,000 plaques of both libraries were separated from spinach using a rabbit antiserum directed against pure SPS protein in relation to immunologically cross reacting protein. (Sonnewald et al., 1992, in press). From the potato library, positively reacting clones were obtained. These clones were further purified by standard methods and, by in vivo excision, plasmids were obtained which carried a double stranded cDNA as an insertion. After testing the size of the insertions, individual clones were analysed by determining the primary sequence.

Example 2

Determination of the nucleotide sequence of the SPS from potato coding cDNA molecules and derivation of the corresponding amino acid sequences

The nucleotide sequences of the insertions obtained from Example 1 were determined by standard methods by means of the dideoxy method (Sanger et al. (1977) Proc. Natl. Acad. Sci. USA, 74, 5463-5467). The nucleotide sequences (Seq. ID No. 1 to Seq. ID No. 3) are described above. The amino acid sequences derived therefrom are also given.

Example 3

Construct of the plasmid p35s-anti-pot-SPS and insertion of gene 35s-anti-pot-sps in the genome of potato plants

The gene 35s-anti-pot-SPS consists of the three fragments A, B and C (see FIG. 1).

The plasmid was prepared as follows: From the pBluescript plasmid with the total insertion, an approximately 2 kb size fragment was prepared by EcoRV cleavage, and this was cloned in the SmaI cleavage site of the vector pBinAR (Hofgen & Willmitzer, 1990, Plant Sci., 66, 221-230). The vector pBinAR is a derivative of the binary vector BIN 19 (Bevan, 1984, Nucl. Acids Res. 12: 8711-8721) and was transferred using an Agrobacterium tumefaciens mediated transformation into potato. Intact, fertile plants were regenerated from the transformed cells.

As a result of the transformation, some transgenic potato plants were shown to have a reduced amount of RNA coding for the potato SPS (see FIG. 3). 50 μg total RNA in a Northern blot experiment was hybridised with the probe for SPS from potato.

Further the plants showed a reduction in SPS activity (see Table I).

Thus, by the transfer and expression of the gene 35s-anti-pot-SPS in potato plants, the amount of mRNA for the SPS protein which is formed, as well as the existing enzymatic activity can be significantly reduced.

Example 4

Construct of plasmid pB33-anti-pot-SPS and insertion of gene B33-anti-pot-SPS in the renome of potato plants

The gene B33-anti-pot-SPS consists of the three fragments A, B and C (see FIG. 4). The plasmid was prepared in an analogous method to that described in Example 3, except that a pBin 19 derivative was used as starting vector which contains the B33 promoter of the patatin gene from Solanum tuberosum (Rocha-Sosa et al., 1989, EMBO J. 8: 23-29) in place of the 35S promoter of pBinAR.

The gene B33-anti-pot-SPS was transferred into potato plants using an Agrobacterium tumefaciens mediated transformation. Intact, fertile plants were regenerated from the transformed cells.

As a result of the transformation, some transgenic potato plants were shown with a reduced amount of RNA coding for the potato SPS (see FIG. 4). 50 μg total RNA in a Northern blot experiment was hybridised with the probe for SPS from potato.

Further the plants also showed a reduction of the SPS activity only in the tubers.

Thus, by the transfer and expression of the gene 35s-anti-pot-SPS in potato plants, the amount of mRNA for the SPS protein which is formed, as well as the existing enzymatic activity can be significantly reduced.

                  TABLE I                                                          ______________________________________                                         Results of the transformation of potato plants                                 1          2      3          4    5                                            ______________________________________                                         Control    26.1   3.6        13.8 100                                          1-55       11.8   2.7        22.9 45                                           1-57       20.4   5.9        28.9 78                                           1-59        3.8   1.4        36.8 14.6                                         1-67        3.8   1.7        44.7 14.6                                         1-69       17.2   2.0        11.7 67                                           1-72       14.6   1.9        13.0 56                                           1-74        5.1   1.7        33.3 19.5                                         ______________________________________                                          Column 1: Control = Wild type plants, numbers indicate individual              transgenic plants                                                              Column 2: Maximal speed of the enzyme reaction in the SPS activity test i      nmol/min/mg.                                                                   Column 3: Speed in the SPS activity test in nmol/min/mg.                       Column 4: Activity level of the SPS in %.                                      Column 5: Residual activity of the SPS in %.                             

    __________________________________________________________________________     SEQUENCE LISTING                                                               (1) GENERAL INFORMATION:                                                       (iii) NUMBER OF SEQUENCES: 6                                                   (2) INFORMATION FOR SEQ ID NO:1:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 3740 base pairs                                                    (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: cDNA                                                       (vi) ORIGINAL SOURCE:                                                          (A) ORGANISM: Solanum tuberosum                                                (ix) FEATURE:                                                                  (A) NAME/KEY: CDS                                                              (B) LOCATION: 957..3494                                                        (D) OTHER INFORMATION: /note= "Sucrose-Phosphate-Synthase"                     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:                                        CTATTCTCTCCCCTCCTTTTTCTCCTCTCTTCAACCCCAAAACTTCCCTTTCAAAGCCTT60                 TGCTTTCCCTTTCTCACTTACCCAGATCAACTAAGCCAATTTGCTGTAGCCTCAGAAAAC120                AGCATTCCCAGATTGAAAAAGAATCTTTTTCAGTACCCAAAAGTTGGGTTTCTCATGTCC180                AGCAAGGATTAGCTGCTCTAGCTATTTCTTTAGCCCTTAATTTTTGTCCAGTTGTGTCTT240                CTGATTCTGCATTGGCATCTGAATTTGATGTGTTAAATGAAGGGCCACCAAAGGACTCAT300                ATGTAGTTGATGATGCTGGTGTGCTTAGCAGGGTGACAAAGTCTGATTTGAAGGCATTGT360                TGTCTGATGTGGAGAAGAGAAAAGGCTTCCACATTAATTTCATCACTGTCCGCAAGCTCA420                CTAGCAAAGCTGATGCTTTTGAGTATGCTGACCAAGTTTTGGAGAAGTGGTACCCTAGTG480                TTGAACAAGGAAATGATAAGGGTATAGTTGTGCTTGTTACAAGTCAAAAGGAAGGCGCAA540                TAACCGGTGGCCCTGATTTTGTAAAGGCCGTTGGAGATACTGTTCTTGATGCTACCGTCT600                CAGAGAACCTTCCAGTGTTGGCTACTGAAGAGAAGTACAATGAAGCAGTTTTCAGCACTG660                CCACACGTCTTGTTGCAGCCATTGATGGCCTTCCTGATCCTGGTGGACCCCAACTCAAGG720                ATAACAAAAGAGAGTCCAACTTCAAATCCAGAGAGGAAACTGATGAGAAAAGAGGACAAT780                TCACACTTGTGGTTGGTGGGCTGTTAGTGATTGCTTTTGTTGTTCCTATGGCTCAATACT840                ATGCATATGTTTCAAAGAAGTGAACTGTCTGATTCTGGAAAGTTACATTTTCGTGAGATT900                TGAGTAAGCATGTATATTATCGTGTACAAAATGGTCCATTCGGAAATGACTGATTC956                    ATGAGATATTTAAAAAGGATAAATATGAAGATTTGGACCTCCCCTAAC1004                           MetArgTyrLeuLysArgIleAsnMetLysIleTrpThrSerProAsn                               151015                                                                         ATAACGGATACTGCCATTTCTTTTTCAGAGATGCTGACGCCAATAAGT1052                           IleThrAspThrAlaIleSerPheSerGluMetLeuThrProIleSer                               202530                                                                         ACAGACGGCTTGATGACTGAGATGGGGGAGAGTAGTGGTGCTTATATT1100                           ThrAspGlyLeuMetThrGluMetGlyGluSerSerGlyAlaTyrIle                               354045                                                                         ATTCGCATTCCTTTTGGACCAAGAGAGAAATATATTCCAAAAGAACAG1148                           IleArgIleProPheGlyProArgGluLysTyrIleProLysGluGln                               505560                                                                         CTATGGCCCTATATTCCCGAATTTGTTGATGGTGCACTTAACCATATT1196                           LeuTrpProTyrIleProGluPheValAspGlyAlaLeuAsnHisIle                               65707580                                                                       ATTCAAATGTCCAAAGTTCTTGGGGAGCAAATTGGTAGTGGCTATCCT1244                           IleGlnMetSerLysValLeuGlyGluGlnIleGlySerGlyTyrPro                               859095                                                                         GTGTGGCCTGTTGCCATACACGGACATTATGCTGATGCTGGCGACTCA1292                           ValTrpProValAlaIleHisGlyHisTyrAlaAspAlaGlyAspSer                               100105110                                                                      GCTGCTCTCCTGTCAGGTGCTTTAAATGTACCAATGCTTTTCACTGGT1340                           AlaAlaLeuLeuSerGlyAlaLeuAsnValProMetLeuPheThrGly                               115120125                                                                      CACTCACTTGGTAGAGATAAGTTGGAGCAACTGTTGCGACAAGGTCGT1388                           HisSerLeuGlyArgAspLysLeuGluGlnLeuLeuArgGlnGlyArg                               130135140                                                                      TTGTCAAAGGATGAAATAAACTCAACCTACAAGATAATGCGGAGAATA1436                           LeuSerLysAspGluIleAsnSerThrTyrLysIleMetArgArgIle                               145150155160                                                                   GAGGCTGAAGAATTAACTCTTGATGCTTCCGAAATTGTCATCACTAGT1484                           GluAlaGluGluLeuThrLeuAspAlaSerGluIleValIleThrSer                               165170175                                                                      ACAAGACAGGAGATTGACGAGCAATGGCGTTTGTATGATGGGTTTGAT1532                           ThrArgGlnGluIleAspGluGlnTrpArgLeuTyrAspGlyPheAsp                               180185190                                                                      CCAATATTAGAGCGTAAGTTACGTGCAAGGATCAAGCGCAATGTCAGC1580                           ProIleLeuGluArgLysLeuArgAlaArgIleLysArgAsnValSer                               195200205                                                                      TGTTATGGCAGGTTTATGCCTCGTATGGCTGTAATTCCTCCTGGGATG1628                           CysTyrGlyArgPheMetProArgMetAlaValIleProProGlyMet                               210215220                                                                      GAGTTCCACCATATTGTGCCACATGAAGGTGACATGGATGGAGAAACA1676                           GluPheHisHisIleValProHisGluGlyAspMetAspGlyGluThr                               225230235240                                                                   GAAGGAAGTGAAGATGGGAAGACCCCGGATCCACCTATTTGGGCAGAG1724                           GluGlySerGluAspGlyLysThrProAspProProIleTrpAlaGlu                               245250255                                                                      ATTATGCGCTTCTTTTCTAATCCAAGGAAGCCTATGATACTCGCACTT1772                           IleMetArgPhePheSerAsnProArgLysProMetIleLeuAlaLeu                               260265270                                                                      GCTAGGCCTGATCCCAAGAAGAACCTCACTACTTTAGTGAAAGCATTT1820                           AlaArgProAspProLysLysAsnLeuThrThrLeuValLysAlaPhe                               275280285                                                                      GGTGAATGTCGTCCATTGAGAGAGCTTGCTAATCTTACTTTGATAATG1868                           GlyGluCysArgProLeuArgGluLeuAlaAsnLeuThrLeuIleMet                               290295300                                                                      GGTAATCGAGATAATATCGACGAAATGTCTAGCACCAATTCTGCACTT1916                           GlyAsnArgAspAsnIleAspGluMetSerSerThrAsnSerAlaLeu                               305310315320                                                                   CTTCTTTCAATCTTGAAAATGATAGATAAGTATGATCTTTATGGTCAA1964                           LeuLeuSerIleLeuLysMetIleAspLysTyrAspLeuTyrGlyGln                               325330335                                                                      GTAGCTTATCCTAAACACCACAAGCAGTCAGATGTTCCTGATATCTAC2012                           ValAlaTyrProLysHisHisLysGlnSerAspValProAspIleTyr                               340345350                                                                      CGTCTTGCTGCAAAGACTAAGGGTGTTTTTATTAATCCAGCTTTTATT2060                           ArgLeuAlaAlaLysThrLysGlyValPheIleAsnProAlaPheIle                               355360365                                                                      GAGCCTTTTGGACTGACTTTGATTGAGGCAGCAGCTTATGGTCTCCCA2108                           GluProPheGlyLeuThrLeuIleGluAlaAlaAlaTyrGlyLeuPro                               370375380                                                                      ATGGTAGCCACAAAAAATGGAGGACCTGTTGATATACATAGGGTTCTT2156                           MetValAlaThrLysAsnGlyGlyProValAspIleHisArgValLeu                               385390395400                                                                   GACAATGGTCTCTTAGTGGATCCCCATGATCAGCAGGCAATTGCTGAT2204                           AspAsnGlyLeuLeuValAspProHisAspGlnGlnAlaIleAlaAsp                               405410415                                                                      GCTCTTTTGAAGTTGGTTGCTGATAAGCAACTGTGGGCTAAATGCAGG2252                           AlaLeuLeuLysLeuValAlaAspLysGlnLeuTrpAlaLysCysArg                               420425430                                                                      GCAAATGGATTAAAAAATATCCACCTTTTCTCATGGCCCGAGCACTGT2300                           AlaAsnGlyLeuLysAsnIleHisLeuPheSerTrpProGluHisCys                               435440445                                                                      AAAACTTATCTATCCCGGATAGCTAGCTGCAAACCAAGGCAACCACGC2348                           LysThrTyrLeuSerArgIleAlaSerCysLysProArgGlnProArg                               450455460                                                                      TGGCTGAGATCCATTGATGATGATGATGAAAATTCAGAAACAGATTCA2396                           TrpLeuArgSerIleAspAspAspAspGluAsnSerGluThrAspSer                               465470475480                                                                   CCTAGTGATTCCTTGAGAGATATTCATGATATATCTCTGAATTTGAGA2444                           ProSerAspSerLeuArgAspIleHisAspIleSerLeuAsnLeuArg                               485490495                                                                      TTTTCATTAGATGGGGAAAAGAATGACAATAAAGAAAATGCTGATAAT2492                           PheSerLeuAspGlyGluLysAsnAspAsnLysGluAsnAlaAspAsn                               500505510                                                                      ACATTAGACCCCGAAGTTCGAAGGAGCAAGTTAGAGAATGCTGTTTTG2540                           ThrLeuAspProGluValArgArgSerLysLeuGluAsnAlaValLeu                               515520525                                                                      TCCTTATCTAAGGGTGCACTGAAGAGCACATCAAAATCTTGGTCGTCA2588                           SerLeuSerLysGlyAlaLeuLysSerThrSerLysSerTrpSerSer                               530535540                                                                      GACAAGGCAGACCAAAACCCTGGTGCTGGTAAATTCCCAGCGATTAGG2636                           AspLysAlaAspGlnAsnProGlyAlaGlyLysPheProAlaIleArg                               545550555560                                                                   AGGAGGCGACATATTTTTGTTATTGCAGTGGATTGTGATGCTAGCTCA2684                           ArgArgArgHisIlePheValIleAlaValAspCysAspAlaSerSer                               565570575                                                                      GGACTCTCTGGAAGTGTGAAAAAGATATTTGAGGCTGTAGAGAAGGAA2732                           GlyLeuSerGlySerValLysLysIlePheGluAlaValGluLysGlu                               580585590                                                                      AGGGCAGAGGGTTCCATTGGATTTATCCTGGCTACATCTTTCAATATA2780                           ArgAlaGluGlySerIleGlyPheIleLeuAlaThrSerPheAsnIle                               595600605                                                                      TCAGAAGTACAGTCTTTCCTGCTTTCAGAGGGCATGAATCCTACTGAT2828                           SerGluValGlnSerPheLeuLeuSerGluGlyMetAsnProThrAsp                               610615620                                                                      TTTGATGCTTACATATGCAATAGTGGTGGTGATCTTTATTATTCGTCC2876                           PheAspAlaTyrIleCysAsnSerGlyGlyAspLeuTyrTyrSerSer                               625630635640                                                                   TTCCATTCTGAGCAAAATCCTTTTGTAGTTGACTTGTACTATCACTCA2924                           PheHisSerGluGlnAsnProPheValValAspLeuTyrTyrHisSer                               645650655                                                                      CATATTGAGTATCGTTGGGGGGGCGAAGGATTGAGAAAGACTTTGGTG2972                           HisIleGluTyrArgTrpGlyGlyGluGlyLeuArgLysThrLeuVal                               660665670                                                                      CGTTGGGCCGCCTCTATCATTGATAAGAATGGTGAAAATGGAGATCAC3020                           ArgTrpAlaAlaSerIleIleAspLysAsnGlyGluAsnGlyAspHis                               675680685                                                                      ATTGTTGTTGAGGATGAAGACAATTCAGCTGACTACTGCTATACTTTC3068                           IleValValGluAspGluAspAsnSerAlaAspTyrCysTyrThrPhe                               690695700                                                                      AAAGTCTGCAAGCCTGGGACGGTTCCTCCATCTAAAGAGCTTAGAAAA3116                           LysValCysLysProGlyThrValProProSerLysGluLeuArgLys                               705710715720                                                                   GTAATGCGAATTCAGGCACTTCGTTGTCACGCTGTTTATTGTCAAAAT3164                           ValMetArgIleGlnAlaLeuArgCysHisAlaValTyrCysGlnAsn                               725730735                                                                      GGGAGTAGGATTAATGTGATCCCTGTACTGGCATCTCGGTCCCAAGCA3212                           GlySerArgIleAsnValIleProValLeuAlaSerArgSerGlnAla                               740745750                                                                      CTCAGGTACTTATATCTGCGATGGGGAATGGACTTGTCGAAGTTGGTG3260                           LeuArgTyrLeuTyrLeuArgTrpGlyMetAspLeuSerLysLeuVal                               755760765                                                                      GTTTTCGTCGGAGAAAGTGGTGATACCGATTATGAAGGATTAATCGGT3308                           ValPheValGlyGluSerGlyAspThrAspTyrGluGlyLeuIleGly                               770775780                                                                      GGTCTACGCAAGGCTGTCATAATGAAAGGCCTCTGCACTAATGCAAGC3356                           GlyLeuArgLysAlaValIleMetLysGlyLeuCysThrAsnAlaSer                               785790795800                                                                   AGCTTAATTCACGGTAATAGGAATTACCCGCTATCTGATGTTTTACCA3404                           SerLeuIleHisGlyAsnArgAsnTyrProLeuSerAspValLeuPro                               805810815                                                                      TTCGACAGCCCTAATGTCATCCAAGCGGACGAGGAATGTAGCAGCACC3452                           PheAspSerProAsnValIleGlnAlaAspGluGluCysSerSerThr                               820825830                                                                      GAAATCCGTTGCTTACTGGTGAAACTAGCGGTACTCAAAGGA3494                                 GluIleArgCysLeuLeuValLysLeuAlaValLeuLysGly                                     835840845                                                                      TAATACCCTTCCCCCTTTGATTGTCAAAAACCTATATGAGCTATAAGACTATGCCATGAA3554               AAGAATGGCCATCCATTTGGCTTGTCTTTTGAAGCTGTTAATACTTTTCAACAGACTACA3614               AAATGAGATGAGTCCTTTGATCCTCTTTAAAGGACATAAAAGCTTTATGCAAGAACCAGT3674               GCTGTAAAGTTATAGAATTTCTTTTGCTATATATGACATTCGACAGAACCTGTTCCGGTT3734               CATCGA3740                                                                     (2) INFORMATION FOR SEQ ID NO:2:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 846 amino acids                                                    (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: protein                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:                                        MetArgTyrLeuLysArgIleAsnMetLysIleTrpThrSerProAsn                               151015                                                                         IleThrAspThrAlaIleSerPheSerGluMetLeuThrProIleSer                               202530                                                                         ThrAspGlyLeuMetThrGluMetGlyGluSerSerGlyAlaTyrIle                               354045                                                                         IleArgIleProPheGlyProArgGluLysTyrIleProLysGluGln                               505560                                                                         LeuTrpProTyrIleProGluPheValAspGlyAlaLeuAsnHisIle                               65707580                                                                       IleGlnMetSerLysValLeuGlyGluGlnIleGlySerGlyTyrPro                               859095                                                                         ValTrpProValAlaIleHisGlyHisTyrAlaAspAlaGlyAspSer                               100105110                                                                      AlaAlaLeuLeuSerGlyAlaLeuAsnValProMetLeuPheThrGly                               115120125                                                                      HisSerLeuGlyArgAspLysLeuGluGlnLeuLeuArgGlnGlyArg                               130135140                                                                      LeuSerLysAspGluIleAsnSerThrTyrLysIleMetArgArgIle                               145150155160                                                                   GluAlaGluGluLeuThrLeuAspAlaSerGluIleValIleThrSer                               165170175                                                                      ThrArgGlnGluIleAspGluGlnTrpArgLeuTyrAspGlyPheAsp                               180185190                                                                      ProIleLeuGluArgLysLeuArgAlaArgIleLysArgAsnValSer                               195200205                                                                      CysTyrGlyArgPheMetProArgMetAlaValIleProProGlyMet                               210215220                                                                      GluPheHisHisIleValProHisGluGlyAspMetAspGlyGluThr                               225230235240                                                                   GluGlySerGluAspGlyLysThrProAspProProIleTrpAlaGlu                               245250255                                                                      IleMetArgPhePheSerAsnProArgLysProMetIleLeuAlaLeu                               260265270                                                                      AlaArgProAspProLysLysAsnLeuThrThrLeuValLysAlaPhe                               275280285                                                                      GlyGluCysArgProLeuArgGluLeuAlaAsnLeuThrLeuIleMet                               290295300                                                                      GlyAsnArgAspAsnIleAspGluMetSerSerThrAsnSerAlaLeu                               305310315320                                                                   LeuLeuSerIleLeuLysMetIleAspLysTyrAspLeuTyrGlyGln                               325330335                                                                      ValAlaTyrProLysHisHisLysGlnSerAspValProAspIleTyr                               340345350                                                                      ArgLeuAlaAlaLysThrLysGlyValPheIleAsnProAlaPheIle                               355360365                                                                      GluProPheGlyLeuThrLeuIleGluAlaAlaAlaTyrGlyLeuPro                               370375380                                                                      MetValAlaThrLysAsnGlyGlyProValAspIleHisArgValLeu                               385390395400                                                                   AspAsnGlyLeuLeuValAspProHisAspGlnGlnAlaIleAlaAsp                               405410415                                                                      AlaLeuLeuLysLeuValAlaAspLysGlnLeuTrpAlaLysCysArg                               420425430                                                                      AlaAsnGlyLeuLysAsnIleHisLeuPheSerTrpProGluHisCys                               435440445                                                                      LysThrTyrLeuSerArgIleAlaSerCysLysProArgGlnProArg                               450455460                                                                      TrpLeuArgSerIleAspAspAspAspGluAsnSerGluThrAspSer                               465470475480                                                                   ProSerAspSerLeuArgAspIleHisAspIleSerLeuAsnLeuArg                               485490495                                                                      PheSerLeuAspGlyGluLysAsnAspAsnLysGluAsnAlaAspAsn                               500505510                                                                      ThrLeuAspProGluValArgArgSerLysLeuGluAsnAlaValLeu                               515520525                                                                      SerLeuSerLysGlyAlaLeuLysSerThrSerLysSerTrpSerSer                               530535540                                                                      AspLysAlaAspGlnAsnProGlyAlaGlyLysPheProAlaIleArg                               545550555560                                                                   ArgArgArgHisIlePheValIleAlaValAspCysAspAlaSerSer                               565570575                                                                      GlyLeuSerGlySerValLysLysIlePheGluAlaValGluLysGlu                               580585590                                                                      ArgAlaGluGlySerIleGlyPheIleLeuAlaThrSerPheAsnIle                               595600605                                                                      SerGluValGlnSerPheLeuLeuSerGluGlyMetAsnProThrAsp                               610615620                                                                      PheAspAlaTyrIleCysAsnSerGlyGlyAspLeuTyrTyrSerSer                               625630635640                                                                   PheHisSerGluGlnAsnProPheValValAspLeuTyrTyrHisSer                               645650655                                                                      HisIleGluTyrArgTrpGlyGlyGluGlyLeuArgLysThrLeuVal                               660665670                                                                      ArgTrpAlaAlaSerIleIleAspLysAsnGlyGluAsnGlyAspHis                               675680685                                                                      IleValValGluAspGluAspAsnSerAlaAspTyrCysTyrThrPhe                               690695700                                                                      LysValCysLysProGlyThrValProProSerLysGluLeuArgLys                               705710715720                                                                   ValMetArgIleGlnAlaLeuArgCysHisAlaValTyrCysGlnAsn                               725730735                                                                      GlySerArgIleAsnValIleProValLeuAlaSerArgSerGlnAla                               740745750                                                                      LeuArgTyrLeuTyrLeuArgTrpGlyMetAspLeuSerLysLeuVal                               755760765                                                                      ValPheValGlyGluSerGlyAspThrAspTyrGluGlyLeuIleGly                               770775780                                                                      GlyLeuArgLysAlaValIleMetLysGlyLeuCysThrAsnAlaSer                               785790795800                                                                   SerLeuIleHisGlyAsnArgAsnTyrProLeuSerAspValLeuPro                               805810815                                                                      PheAspSerProAsnValIleGlnAlaAspGluGluCysSerSerThr                               820825830                                                                      GluIleArgCysLeuLeuValLysLeuAlaValLeuLysGly                                     835840845                                                                      (2) INFORMATION FOR SEQ ID NO:3:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 3625 base pairs                                                    (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: cDNA                                                       (vi) ORIGINAL SOURCE:                                                          (A) ORGANISM: Solanum tuberosum                                                (ix) FEATURE:                                                                  (A) NAME/KEY: CDS                                                              (B) LOCATION: 121..3282                                                        (D) OTHER INFORMATION: /note= "Sucrose-Phosphate-Synthase"                     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:                                        ATTTTTTTCTCTAAGTTCTCTCTCGCTGTCCTTATCATTTCACCACCTCCATAAATCTAG60                 AAACATCTTTTCTACTCCGTTAATCTCTCTAGCACACGGCGGAGGAGTGCGGCGGAGGAG120                ATGGCGGGAAACGATTGGATTAACAGTTACTTAGAGGCGATACTGGAT168                            MetAlaGlyAsnAspTrpIleAsnSerTyrLeuGluAlaIleLeuAsp                               151015                                                                         GTTGGACCAGGGCTAGATGATAAGAAGTCATCGTTGTTGTTGAGAGAA216                            ValGlyProGlyLeuAspAspLysLysSerSerLeuLeuLeuArgGlu                               202530                                                                         AGAGGGAGGTTTAGTCCGACGAGGTACTTTGTTGAGGAAGTTATTACT264                            ArgGlyArgPheSerProThrArgTyrPheValGluGluValIleThr                               354045                                                                         GGATTCGATGAGACTGATTTGCATCGTTCGTGGATCCGAGCACAAGCT312                            GlyPheAspGluThrAspLeuHisArgSerTrpIleArgAlaGlnAla                               505560                                                                         ACTCGGAGTCCGCAGAGAAGGAATACTAGGCTCGAGAATATGTGCTGG360                            ThrArgSerProGlnArgArgAsnThrArgLeuGluAsnMetCysTrp                               65707580                                                                       AGGATTTGGAATTTGGCTCGCCAGAAAAAGCAGCTTGAGGGAGAGCAA408                            ArgIleTrpAsnLeuAlaArgGlnLysLysGlnLeuGluGlyGluGln                               859095                                                                         GCTCAGTGGATGGCAAAACGCCGTCAAGAACGTGAAAGAGGTCGCAGA456                            AlaGlnTrpMetAlaLysArgArgGlnGluArgGluArgGlyArgArg                               100105110                                                                      GAAGCAGTTGCTGATATGTCAGAGGATCTATCTGAGGGAGAGAAAGGA504                            GluAlaValAlaAspMetSerGluAspLeuSerGluGlyGluLysGly                               115120125                                                                      GATATAGTCGCTGACATGTCATCTCATGGTGAAAGTACCAGAGGCCGA552                            AspIleValAlaAspMetSerSerHisGlyGluSerThrArgGlyArg                               130135140                                                                      TTGCCTAGAATCAGTTCTGTTGAGACAATGGAAGCATGGGTCAGTCAG600                            LeuProArgIleSerSerValGluThrMetGluAlaTrpValSerGln                               145150155160                                                                   CAGAGAGGAAAGAAGCTTTATATCGTGCTTATAAGTTTACATGGTTTA648                            GlnArgGlyLysLysLeuTyrIleValLeuIleSerLeuHisGlyLeu                               165170175                                                                      ATTCGGGGTGAGAATATGGAGCTTGGACGGGATTCTGATACTGGTGGT696                            IleArgGlyGluAsnMetGluLeuGlyArgAspSerAspThrGlyGly                               180185190                                                                      CAGGTGAAGTATGTTGTTGAACTTGCGAGGGCCTTAGGGTCGATGCCA744                            GlnValLysTyrValValGluLeuAlaArgAlaLeuGlySerMetPro                               195200205                                                                      GGTGTATATCGGGTTGACTTGCTTACTAGACAAGTATCTTCACCAGAA792                            GlyValTyrArgValAspLeuLeuThrArgGlnValSerSerProGlu                               210215220                                                                      GTAGATTGGAGCTATGGTGAGCCGACAGAGATGCTGACGCCAATAAGT840                            ValAspTrpSerTyrGlyGluProThrGluMetLeuThrProIleSer                               225230235240                                                                   ACAGACGGCTTGATGACTGAGATGGGGGAGAGTAGTGGTGCTTATATT888                            ThrAspGlyLeuMetThrGluMetGlyGluSerSerGlyAlaTyrIle                               245250255                                                                      ATTCGCATTCCTTTTGGACCAAGAGAGAAATATATTCCAAAAGAACAG936                            IleArgIleProPheGlyProArgGluLysTyrIleProLysGluGln                               260265270                                                                      CTATGGCCCTATATTCCCGAATTTGTTGATGGTGCACTTAACCATATT984                            LeuTrpProTyrIleProGluPheValAspGlyAlaLeuAsnHisIle                               275280285                                                                      ATTCAAATGTCCAAAGTTCTTGGGGAGCAAATTGGTAGTGGCTATCCT1032                           IleGlnMetSerLysValLeuGlyGluGlnIleGlySerGlyTyrPro                               290295300                                                                      GTGTGGCCTGTTGCCATACACGGACATTATGCTGATGCTGGCGACTCA1080                           ValTrpProValAlaIleHisGlyHisTyrAlaAspAlaGlyAspSer                               305310315320                                                                   GCTGCTCTCCTGTCAGGTGCTTTAAATGTACCAATGCTTTTCACTGGT1128                           AlaAlaLeuLeuSerGlyAlaLeuAsnValProMetLeuPheThrGly                               325330335                                                                      CACTCACTTGGTAGAGATAAGTTGGAGCAACTGTTGGCACAAGGTCGA1176                           HisSerLeuGlyArgAspLysLeuGluGlnLeuLeuAlaGlnGlyArg                               340345350                                                                      AAGTCAAAGGATGAAATAAACTCAACCTACAAGATAATGCGGAGAATA1224                           LysSerLysAspGluIleAsnSerThrTyrLysIleMetArgArgIle                               355360365                                                                      GAGGCTGAAGAATTAACTCTTGATGCTTCCGAAATTGTCATCACTAGT1272                           GluAlaGluGluLeuThrLeuAspAlaSerGluIleValIleThrSer                               370375380                                                                      ACAAGACAGGAGATTGACGAGCAATGGCGTTTGTATGATGGGTTTGAT1320                           ThrArgGlnGluIleAspGluGlnTrpArgLeuTyrAspGlyPheAsp                               385390395400                                                                   CCAATATTAGAGCGTAAGTTACGTGCAAGGATCAAGCGCAATGTCAGC1368                           ProIleLeuGluArgLysLeuArgAlaArgIleLysArgAsnValSer                               405410415                                                                      TGTTATGGCAGGTTTATGCCTCGTATGGCTGTAATTCCTCCTGGGATG1416                           CysTyrGlyArgPheMetProArgMetAlaValIleProProGlyMet                               420425430                                                                      GAGTTCCACCATATTGTGCCACATGAAGGTGACATGGATGGTGAAACA1464                           GluPheHisHisIleValProHisGluGlyAspMetAspGlyGluThr                               435440445                                                                      GAAGGAAGTGAAGATGGGAAGACCCCGGATCCACCTATTTGGGCAGAG1512                           GluGlySerGluAspGlyLysThrProAspProProIleTrpAlaGlu                               450455460                                                                      ATTATGCGCTTCTTTTCTAATCCAAGGAAGCCTATGATACTCGCACTT1560                           IleMetArgPhePheSerAsnProArgLysProMetIleLeuAlaLeu                               465470475480                                                                   GCTAGGCCTGATCCCAAGAAGAACCTCACTACTTTAGTGAAAGCATTT1608                           AlaArgProAspProLysLysAsnLeuThrThrLeuValLysAlaPhe                               485490495                                                                      GGTGAATGTCGTCCATTGAGAGAGCTTGCTAATCTTACTTTGATAATG1656                           GlyGluCysArgProLeuArgGluLeuAlaAsnLeuThrLeuIleMet                               500505510                                                                      GGTAATCGAGATAATATCGACGAAATGTCTAGCACCAATTCTGCACTT1704                           GlyAsnArgAspAsnIleAspGluMetSerSerThrAsnSerAlaLeu                               515520525                                                                      CTTCTTTCAATCTTGAAAATGATAGATAAGTATGATCTTTATGGTCAA1752                           LeuLeuSerIleLeuLysMetIleAspLysTyrAspLeuTyrGlyGln                               530535540                                                                      GTAGCTTATCCTAAACACCACAAGCAGTCAGATGTTCCTGATATCTAC1800                           ValAlaTyrProLysHisHisLysGlnSerAspValProAspIleTyr                               545550555560                                                                   CGTCTTGCTGCAAAGACTAAGGGTGTTTTTATTAATCCAGCTTTTATT1848                           ArgLeuAlaAlaLysThrLysGlyValPheIleAsnProAlaPheIle                               565570575                                                                      GAGCCTTTTGGACTGACTTTGATTGAGGCAGCAGCTTATGGTCTCCCA1896                           GluProPheGlyLeuThrLeuIleGluAlaAlaAlaTyrGlyLeuPro                               580585590                                                                      ATGGTAGCCACAAAAAATGGAGGACCTGTTGATATACATAGGGTTCTT1944                           MetValAlaThrLysAsnGlyGlyProValAspIleHisArgValLeu                               595600605                                                                      GACAATGGTCTCTTAGTGGATCCCCATGATCAGCAGGCAATTGCTGAT1992                           AspAsnGlyLeuLeuValAspProHisAspGlnGlnAlaIleAlaAsp                               610615620                                                                      GCTCTTTTGAAGTTGGTTGCTGATAAGCAACTGTGGGCTAAATGCAGG2040                           AlaLeuLeuLysLeuValAlaAspLysGlnLeuTrpAlaLysCysArg                               625630635640                                                                   GCAAATGGATTAAAAAATATCCACCTTTTCTCATGGCCCGAGCACTGT2088                           AlaAsnGlyLeuLysAsnIleHisLeuPheSerTrpProGluHisCys                               645650655                                                                      AAAACTTATCTATCCCGGATAGCTAGCTGCAAACCAAGGCAACCACGC2136                           LysThrTyrLeuSerArgIleAlaSerCysLysProArgGlnProArg                               660665670                                                                      TGGCTGAGATCCATTGATGATGATGATGAAAATTCAGAAACAGATTCA2184                           TrpLeuArgSerIleAspAspAspAspGluAsnSerGluThrAspSer                               675680685                                                                      CCTAGTGATTCCTTGAGAGATATTCATGATATATCTCTGAATTTGAGA2232                           ProSerAspSerLeuArgAspIleHisAspIleSerLeuAsnLeuArg                               690695700                                                                      TTTTCATTAGATGGGGAAAAGAATGACAATAAAGAAAATGCTGATAAT2280                           PheSerLeuAspGlyGluLysAsnAspAsnLysGluAsnAlaAspAsn                               705710715720                                                                   ACATTAGACCCCGAAGTTCGAAGGAGCAAGTTAGAGAATGCTGTTTTG2328                           ThrLeuAspProGluValArgArgSerLysLeuGluAsnAlaValLeu                               725730735                                                                      TCCTTATCTAAGGGTGCACTGAAGAGCACATCAAAATCTTGGTCGTCA2376                           SerLeuSerLysGlyAlaLeuLysSerThrSerLysSerTrpSerSer                               740745750                                                                      GACAAGGCAGACCAAAACCCTGGTGCTGGTAAATTCCCAGCGATTAGG2424                           AspLysAlaAspGlnAsnProGlyAlaGlyLysPheProAlaIleArg                               755760765                                                                      AGGAGGCGACATATTTTTGTTATTGCAGTGGATTGTGATGCTAGCTCA2472                           ArgArgArgHisIlePheValIleAlaValAspCysAspAlaSerSer                               770775780                                                                      GGACTCTCTGGAAGTGTGAAAAAGATATTTGAGGCTGTAGAGAAGGAA2520                           GlyLeuSerGlySerValLysLysIlePheGluAlaValGluLysGlu                               785790795800                                                                   AGGGCAGAGGGTTCCATTGGATTTATCCTGGCTACATCTTTCAATATA2568                           ArgAlaGluGlySerIleGlyPheIleLeuAlaThrSerPheAsnIle                               805810815                                                                      TCAGAAGTACAGTCTTTCCTGCTTTCAGAGGGCATGAATCCTACTGAT2616                           SerGluValGlnSerPheLeuLeuSerGluGlyMetAsnProThrAsp                               820825830                                                                      TTTGATGCTTACATATGCAATAGTGGTGGTGATCTTTATTATTCGTCC2664                           PheAspAlaTyrIleCysAsnSerGlyGlyAspLeuTyrTyrSerSer                               835840845                                                                      TTCCATTCTGAGCAAAATCCTTTTGTAGTTGACTTGTACTATCACTCA2712                           PheHisSerGluGlnAsnProPheValValAspLeuTyrTyrHisSer                               850855860                                                                      CATATTGAGTATCGTTGGGGGGGCGAAGGATTGAGAAAGACTTTGGTG2760                           HisIleGluTyrArgTrpGlyGlyGluGlyLeuArgLysThrLeuVal                               865870875880                                                                   CGTTGGGCCGCCTCTATCATTGATAAGAATGGTGAAAATGGAGATCAC2808                           ArgTrpAlaAlaSerIleIleAspLysAsnGlyGluAsnGlyAspHis                               885890895                                                                      ATTGTTGTTGAGGATGAAGACAATTCAGCTGACTACTGCTATACTTTC2856                           IleValValGluAspGluAspAsnSerAlaAspTyrCysTyrThrPhe                               900905910                                                                      AAAGTCTGCAAGCCTGGGACGGTTCCTCCATCTAAAGAGCTTAGAAAA2904                           LysValCysLysProGlyThrValProProSerLysGluLeuArgLys                               915920925                                                                      GTAATGCGAATTCAGGCACTTCGTTGTCACGCTGTTTATTGTCAAAAT2952                           ValMetArgIleGlnAlaLeuArgCysHisAlaValTyrCysGlnAsn                               930935940                                                                      GGGAGTAGGATTAATGTGATCCCTGTACTGGCATCTCGGTCCCAAGCA3000                           GlySerArgIleAsnValIleProValLeuAlaSerArgSerGlnAla                               945950955960                                                                   CTCAGGTACTTATATCTGCGATGGGGAATGGACTTGTCGAAGTTGGTG3048                           LeuArgTyrLeuTyrLeuArgTrpGlyMetAspLeuSerLysLeuVal                               965970975                                                                      GTTTTCGTCGGAGAAAGTGGTGATACCGATTATGAAGGATTAATCGGT3096                           ValPheValGlyGluSerGlyAspThrAspTyrGluGlyLeuIleGly                               980985990                                                                      GGTCTACGCAAGGCTGTCATAATGAAAGGCCTCTGCACTAATGCAAGC3144                           GlyLeuArgLysAlaValIleMetLysGlyLeuCysThrAsnAlaSer                               99510001005                                                                    AGCTTAATTCACGGTAATAGGAATTACCCGCTATCTGATGTTTTACCA3192                           SerLeuIleHisGlyAsnArgAsnTyrProLeuSerAspValLeuPro                               101010151020                                                                   TTCGACAGCCCTAATGTCATCCAAGCGGACGAGGAATGTAGCAGCACC3240                           PheAspSerProAsnValIleGlnAlaAspGluGluCysSerSerThr                               1025103010351040                                                               GAAATCCGTTGCTTACTGGAGAAACTAGCGGTACTCAAAGGA3282                                 GluIleArgCysLeuLeuGluLysLeuAlaValLeuLysGly                                     10451050                                                                       TAATACCCTTCCCCCTTTGATTGTCAAAAACCTATATGAGCTATAAGACTATGCCATGAA3342               AAGAATGGCCATCCATTTGGCTTGTCTTTTGAAGCTGTTAATACTTTTCAACAGACTACA3402               AAATGAGATGAGTCCTTTGATCCTCTTTAAAGGACATAAAAGCTTTATGCAAGAACCAGT3462               GCTGTAAAGTTATAGAATTTCTTTTGCTATATATGACATTCGACAGAACCAGTTCCGGTT3522               CATCGAGAAAAAGAAATAAATTTCAACTTATAAACATGCCTGATCATGTAAATTATCATA3582               TACATCCATCGGAAGGCATTATCGATGGGTTATCAGATTTTTT3625                                (2) INFORMATION FOR SEQ ID NO:4:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 1054 amino acids                                                   (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: protein                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:                                        MetAlaGlyAsnAspTrpIleAsnSerTyrLeuGluAlaIleLeuAsp                               151015                                                                         ValGlyProGlyLeuAspAspLysLysSerSerLeuLeuLeuArgGlu                               202530                                                                         ArgGlyArgPheSerProThrArgTyrPheValGluGluValIleThr                               354045                                                                         GlyPheAspGluThrAspLeuHisArgSerTrpIleArgAlaGlnAla                               505560                                                                         ThrArgSerProGlnArgArgAsnThrArgLeuGluAsnMetCysTrp                               65707580                                                                       ArgIleTrpAsnLeuAlaArgGlnLysLysGlnLeuGluGlyGluGln                               859095                                                                         AlaGlnTrpMetAlaLysArgArgGlnGluArgGluArgGlyArgArg                               100105110                                                                      GluAlaValAlaAspMetSerGluAspLeuSerGluGlyGluLysGly                               115120125                                                                      AspIleValAlaAspMetSerSerHisGlyGluSerThrArgGlyArg                               130135140                                                                      LeuProArgIleSerSerValGluThrMetGluAlaTrpValSerGln                               145150155160                                                                   GlnArgGlyLysLysLeuTyrIleValLeuIleSerLeuHisGlyLeu                               165170175                                                                      IleArgGlyGluAsnMetGluLeuGlyArgAspSerAspThrGlyGly                               180185190                                                                      GlnValLysTyrValValGluLeuAlaArgAlaLeuGlySerMetPro                               195200205                                                                      GlyValTyrArgValAspLeuLeuThrArgGlnValSerSerProGlu                               210215220                                                                      ValAspTrpSerTyrGlyGluProThrGluMetLeuThrProIleSer                               225230235240                                                                   ThrAspGlyLeuMetThrGluMetGlyGluSerSerGlyAlaTyrIle                               245250255                                                                      IleArgIleProPheGlyProArgGluLysTyrIleProLysGluGln                               260265270                                                                      LeuTrpProTyrIleProGluPheValAspGlyAlaLeuAsnHisIle                               275280285                                                                      IleGlnMetSerLysValLeuGlyGluGlnIleGlySerGlyTyrPro                               290295300                                                                      ValTrpProValAlaIleHisGlyHisTyrAlaAspAlaGlyAspSer                               305310315320                                                                   AlaAlaLeuLeuSerGlyAlaLeuAsnValProMetLeuPheThrGly                               325330335                                                                      HisSerLeuGlyArgAspLysLeuGluGlnLeuLeuAlaGlnGlyArg                               340345350                                                                      LysSerLysAspGluIleAsnSerThrTyrLysIleMetArgArgIle                               355360365                                                                      GluAlaGluGluLeuThrLeuAspAlaSerGluIleValIleThrSer                               370375380                                                                      ThrArgGlnGluIleAspGluGlnTrpArgLeuTyrAspGlyPheAsp                               385390395400                                                                   ProIleLeuGluArgLysLeuArgAlaArgIleLysArgAsnValSer                               405410415                                                                      CysTyrGlyArgPheMetProArgMetAlaValIleProProGlyMet                               420425430                                                                      GluPheHisHisIleValProHisGluGlyAspMetAspGlyGluThr                               435440445                                                                      GluGlySerGluAspGlyLysThrProAspProProIleTrpAlaGlu                               450455460                                                                      IleMetArgPhePheSerAsnProArgLysProMetIleLeuAlaLeu                               465470475480                                                                   AlaArgProAspProLysLysAsnLeuThrThrLeuValLysAlaPhe                               485490495                                                                      GlyGluCysArgProLeuArgGluLeuAlaAsnLeuThrLeuIleMet                               500505510                                                                      GlyAsnArgAspAsnIleAspGluMetSerSerThrAsnSerAlaLeu                               515520525                                                                      LeuLeuSerIleLeuLysMetIleAspLysTyrAspLeuTyrGlyGln                               530535540                                                                      ValAlaTyrProLysHisHisLysGlnSerAspValProAspIleTyr                               545550555560                                                                   ArgLeuAlaAlaLysThrLysGlyValPheIleAsnProAlaPheIle                               565570575                                                                      GluProPheGlyLeuThrLeuIleGluAlaAlaAlaTyrGlyLeuPro                               580585590                                                                      MetValAlaThrLysAsnGlyGlyProValAspIleHisArgValLeu                               595600605                                                                      AspAsnGlyLeuLeuValAspProHisAspGlnGlnAlaIleAlaAsp                               610615620                                                                      AlaLeuLeuLysLeuValAlaAspLysGlnLeuTrpAlaLysCysArg                               625630635640                                                                   AlaAsnGlyLeuLysAsnIleHisLeuPheSerTrpProGluHisCys                               645650655                                                                      LysThrTyrLeuSerArgIleAlaSerCysLysProArgGlnProArg                               660665670                                                                      TrpLeuArgSerIleAspAspAspAspGluAsnSerGluThrAspSer                               675680685                                                                      ProSerAspSerLeuArgAspIleHisAspIleSerLeuAsnLeuArg                               690695700                                                                      PheSerLeuAspGlyGluLysAsnAspAsnLysGluAsnAlaAspAsn                               705710715720                                                                   ThrLeuAspProGluValArgArgSerLysLeuGluAsnAlaValLeu                               725730735                                                                      SerLeuSerLysGlyAlaLeuLysSerThrSerLysSerTrpSerSer                               740745750                                                                      AspLysAlaAspGlnAsnProGlyAlaGlyLysPheProAlaIleArg                               755760765                                                                      ArgArgArgHisIlePheValIleAlaValAspCysAspAlaSerSer                               770775780                                                                      GlyLeuSerGlySerValLysLysIlePheGluAlaValGluLysGlu                               785790795800                                                                   ArgAlaGluGlySerIleGlyPheIleLeuAlaThrSerPheAsnIle                               805810815                                                                      SerGluValGlnSerPheLeuLeuSerGluGlyMetAsnProThrAsp                               820825830                                                                      PheAspAlaTyrIleCysAsnSerGlyGlyAspLeuTyrTyrSerSer                               835840845                                                                      PheHisSerGluGlnAsnProPheValValAspLeuTyrTyrHisSer                               850855860                                                                      HisIleGluTyrArgTrpGlyGlyGluGlyLeuArgLysThrLeuVal                               865870875880                                                                   ArgTrpAlaAlaSerIleIleAspLysAsnGlyGluAsnGlyAspHis                               885890895                                                                      IleValValGluAspGluAspAsnSerAlaAspTyrCysTyrThrPhe                               900905910                                                                      LysValCysLysProGlyThrValProProSerLysGluLeuArgLys                               915920925                                                                      ValMetArgIleGlnAlaLeuArgCysHisAlaValTyrCysGlnAsn                               930935940                                                                      GlySerArgIleAsnValIleProValLeuAlaSerArgSerGlnAla                               945950955960                                                                   LeuArgTyrLeuTyrLeuArgTrpGlyMetAspLeuSerLysLeuVal                               965970975                                                                      ValPheValGlyGluSerGlyAspThrAspTyrGluGlyLeuIleGly                               980985990                                                                      GlyLeuArgLysAlaValIleMetLysGlyLeuCysThrAsnAlaSer                               99510001005                                                                    SerLeuIleHisGlyAsnArgAsnTyrProLeuSerAspValLeuPro                               101010151020                                                                   PheAspSerProAsnValIleGlnAlaAspGluGluCysSerSerThr                               1025103010351040                                                               GluIleArgCysLeuLeuGluLysLeuAlaValLeuLysGly                                     10451050                                                                       (2) INFORMATION FOR SEQ ID NO:5:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 2930 base pairs                                                    (B) TYPE: nucleic acid                                                         (C) STRANDEDNESS: single                                                       (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: cDNA                                                       (vi) ORIGINAL SOURCE:                                                          (A) ORGANISM: Solanum tuberosum                                                (ix) FEATURE:                                                                  (A) NAME/KEY: CDS                                                              (B) LOCATION: 118..2841                                                        (D) OTHER INFORMATION: /note= "Sucrose-Phospahte-Synthase"                     (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:                                        ATTTTTTTCTCTAAATTCTCTCTCACTGTCCTTATCATTTCACCACCTCCATAAATCTAG60                 AAACATCTTTTCTATTCCGTTAATCTCTCTAGCACACGGCGGAGTGCGGCGGAGGAG117                   ATGGCGGGAAACGACTGGATTAACAGTTACTTAGAGGCGATACTGGAT165                            MetAlaGlyAsnAspTrpIleAsnSerTyrLeuGluAlaIleLeuAsp                               151015                                                                         GTAGGACCAGGGCTAGATGATAAGAAATCATCGTTGTTGTTGAGAGAA213                            ValGlyProGlyLeuAspAspLysLysSerSerLeuLeuLeuArgGlu                               202530                                                                         AGAGGGAGGTTTAGTCCGACGAGGTACTTTGTTGAGGAAGTTATTACT261                            ArgGlyArgPheSerProThrArgTyrPheValGluGluValIleThr                               354045                                                                         GGATTCGATGAGACTGATTTGCATCGCTCGTGGATCCGAGCACAAGCT309                            GlyPheAspGluThrAspLeuHisArgSerTrpIleArgAlaGlnAla                               505560                                                                         ACTCGGAGTCCGCAGGAGAGGAATACTAGGCTCGAGAATATGTGCTGG357                            ThrArgSerProGlnGluArgAsnThrArgLeuGluAsnMetCysTrp                               65707580                                                                       AGGATTTGGAATTTGGCTCGCCAGAAAAAGCAGCTTGAGGGAGAGCAA405                            ArgIleTrpAsnLeuAlaArgGlnLysLysGlnLeuGluGlyGluGln                               859095                                                                         GCTCAGTGGATGGCAAAACGCCGTCAAGAACGTGAGAGAGGTCGCAGA453                            AlaGlnTrpMetAlaLysArgArgGlnGluArgGluArgGlyArgArg                               100105110                                                                      GAAGCAGTTGCTGATATGTCAGAGGATCTATCTGAGGGAGAGAAAGGA501                            GluAlaValAlaAspMetSerGluAspLeuSerGluGlyGluLysGly                               115120125                                                                      GATATAGTCGCTGACATGTCATCTCATGGTGAAAGTACCAGAGGCCGA549                            AspIleValAlaAspMetSerSerHisGlyGluSerThrArgGlyArg                               130135140                                                                      TTGCCTAGAATCAGTTCTGTTGAGACAATGGAAGCATGGGTCAGTCAG597                            LeuProArgIleSerSerValGluThrMetGluAlaTrpValSerGln                               145150155160                                                                   CAGAGAGGAAAGAAGCTTTATATCGTGCTTATAAGTTTACATGGTTTA645                            GlnArgGlyLysLysLeuTyrIleValLeuIleSerLeuHisGlyLeu                               165170175                                                                      ATTCGGGGTGAGAATATGGAGCTTGGACGGGATTCTGATACTGGTGGT693                            IleArgGlyGluAsnMetGluLeuGlyArgAspSerAspThrGlyGly                               180185190                                                                      CAGGTGAAGTATGTAGTTGGAGCAACTGTTGCACAAGGTCGTTTGTCA741                            GlnValLysTyrValValGlyAlaThrValAlaGlnGlyArgLeuSer                               195200205                                                                      AAGGATGAAATAAACTCAACCTACAAGATAATGCGGAGAATAGAGGCT789                            LysAspGluIleAsnSerThrTyrLysIleMetArgArgIleGluAla                               210215220                                                                      GAAGAATTAACTCTTGATGCTTCCGAAATTGTCATCACTAGTACAAGA837                            GluGluLeuThrLeuAspAlaSerGluIleValIleThrSerThrArg                               225230235240                                                                   CAGGAGATTGACGAGCAATGGCGTTTGTATGATGGGTTTGATCCAATA885                            GlnGluIleAspGluGlnTrpArgLeuTyrAspGlyPheAspProIle                               245250255                                                                      TTAGAGCGTAAGTTACGTGCAAGGATCAAGCGCAATGTCAGCTGTTAT933                            LeuGluArgLysLeuArgAlaArgIleLysArgAsnValSerCysTyr                               260265270                                                                      GGCAGGTTTATGCCTCGTATGGCTGTAATTCCTCCTGGGATGGAGTTC981                            GlyArgPheMetProArgMetAlaValIleProProGlyMetGluPhe                               275280285                                                                      CACCATATTGTGCCACATGAAGGTGACATGGATGGTGAAACAGAAGGA1029                           HisHisIleValProHisGluGlyAspMetAspGlyGluThrGluGly                               290295300                                                                      AGTGAAGATGGAAAGACCCCGGATCCACCTATTTGGGCAGAGATTATG1077                           SerGluAspGlyLysThrProAspProProIleTrpAlaGluIleMet                               305310315320                                                                   CGCTTCTTTTCTAATCCAAGGAAGCCTATGATACTCGCACTTGCTAGG1125                           ArgPhePheSerAsnProArgLysProMetIleLeuAlaLeuAlaArg                               325330335                                                                      CCTGATCCCAAGAAGAACCTCACTACTTTAGTGAAAGCATTTGGTGAA1173                           ProAspProLysLysAsnLeuThrThrLeuValLysAlaPheGlyGlu                               340345350                                                                      TGTCGTCCATTGAGAGACCTTGCTAATCTTACTTTGATAATGGGTAAT1221                           CysArgProLeuArgAspLeuAlaAsnLeuThrLeuIleMetGlyAsn                               355360365                                                                      CGAGATAATATCGACGAAATGTCTAGCACCAATTCTGCACTTCTTCTT1269                           ArgAspAsnIleAspGluMetSerSerThrAsnSerAlaLeuLeuLeu                               370375380                                                                      TCAATCTTGAAGATGATAGATAAGTATGATCTTTATGGTCTAGTAGCT1317                           SerIleLeuLysMetIleAspLysTyrAspLeuTyrGlyLeuValAla                               385390395400                                                                   TATCCTAAACACCACAAGCAGTCAGATGTTCCTGATATCTACCGTCTT1365                           TyrProLysHisHisLysGlnSerAspValProAspIleTyrArgLeu                               405410415                                                                      GCTGCAAAGACTAAGGGTGTTTTTATTAATCCAGCTTTTATTGAGCCT1413                           AlaAlaLysThrLysGlyValPheIleAsnProAlaPheIleGluPro                               420425430                                                                      TTTGGACTGACTTTGATTGAGGCAGCAGCTTATGGTCTCCCAATGGTA1461                           PheGlyLeuThrLeuIleGluAlaAlaAlaTyrGlyLeuProMetVal                               435440445                                                                      GCCACAAAAAATGGAGGACCTGTTGATATACATAGGGTTCTTGACAAT1509                           AlaThrLysAsnGlyGlyProValAspIleHisArgValLeuAspAsn                               450455460                                                                      GGTCTCTTAGTGGATCCCCATGATCAGCAGGCAATTGCTGATGCTCTT1557                           GlyLeuLeuValAspProHisAspGlnGlnAlaIleAlaAspAlaLeu                               465470475480                                                                   TTGAAGTTGGTTGCTGATAAGCAACTGTGGGCTAAATGCAGGGCAAAT1605                           LeuLysLeuValAlaAspLysGlnLeuTrpAlaLysCysArgAlaAsn                               485490495                                                                      GGATTAAAAAATATCCACCTTTTCTCATGGCCCGAGCACTGTAAAACT1653                           GlyLeuLysAsnIleHisLeuPheSerTrpProGluHisCysLysThr                               500505510                                                                      TATCTATCCCGGATAGCTAGCTGCAAACCGAGGCAACATTCCTTGAGA1701                           TyrLeuSerArgIleAlaSerCysLysProArgGlnHisSerLeuArg                               515520525                                                                      GATATTCATGATATATCTCTGAATTTGAGATTTTCATTAGATGGGGAA1749                           AspIleHisAspIleSerLeuAsnLeuArgPheSerLeuAspGlyGlu                               530535540                                                                      AAGAATGACAATAAAGAAAATGCTGATAATACATTAGACCCCGAAGTT1797                           LysAsnAspAsnLysGluAsnAlaAspAsnThrLeuAspProGluVal                               545550555560                                                                   CGAAGGAGCAAGTTAGAGAATGCTGTTTTGTCCTTATCTAAGGGTGCA1845                           ArgArgSerLysLeuGluAsnAlaValLeuSerLeuSerLysGlyAla                               565570575                                                                      CTGAAGAGCACATCAAAATCTTGGTCGTCAGACAAGGCAGACCAAAAT1893                           LeuLysSerThrSerLysSerTrpSerSerAspLysAlaAspGlnAsn                               580585590                                                                      CCTGGTGCTGGTAAATTCCCAGCGATTAGGAGGAGGCGACATATTTTT1941                           ProGlyAlaGlyLysPheProAlaIleArgArgArgArgHisIlePhe                               595600605                                                                      GTTATTGCAGTGGATTGTGATGCTAGCTCAGGACTCTCTGGAAGTATG1989                           ValIleAlaValAspCysAspAlaSerSerGlyLeuSerGlySerMet                               610615620                                                                      AAAAAGATATTTGAGGCTGTAGAGAAGGAAAGGGCAGAGGGTTCCATT2037                           LysLysIlePheGluAlaValGluLysGluArgAlaGluGlySerIle                               625630635640                                                                   GGATTTATCCTTGCTACATCTTTCAATATATCAGAAGTACAGTCTTTC2085                           GlyPheIleLeuAlaThrSerPheAsnIleSerGluValGlnSerPhe                               645650655                                                                      CTGCTTTCAGAGGGCATGAATCCTACTGAGCAAAATCCTTTTGTAGTT2133                           LeuLeuSerGluGlyMetAsnProThrGluGlnAsnProPheValVal                               660665670                                                                      GACTTGTACTATCACTCACATATTGAGTATCGTTGGGGGGGCGAAGGG2181                           AspLeuTyrTyrHisSerHisIleGluTyrArgTrpGlyGlyGluGly                               675680685                                                                      TTGAGAAAGACTTTGGTGCGTTGGGCCGCCTCTATCATTGATAAGAAT2229                           LeuArgLysThrLeuValArgTrpAlaAlaSerIleIleAspLysAsn                               690695700                                                                      GGTGAAAATGGAGATCACATTGTTGTTGAGGATGAAGACAATTCAGCT2277                           GlyGluAsnGlyAspHisIleValValGluAspGluAspAsnSerAla                               705710715720                                                                   GACTACTGCTATACATTCAAAGTTTGCAAGCCTGGGACGGTTCCTCCA2325                           AspTyrCysTyrThrPheLysValCysLysProGlyThrValProPro                               725730735                                                                      TCTAAAGAACTTAGAAAAGTAATGCGAATTCAGGCACTTCGTTGTCAC2373                           SerLysGluLeuArgLysValMetArgIleGlnAlaLeuArgCysHis                               740745750                                                                      GCTGTTTATTGTCAAAATGGGAGTAGGATTAATGTGATCCCTGTACTG2421                           AlaValTyrCysGlnAsnGlySerArgIleAsnValIleProValLeu                               755760765                                                                      GCATCTCGGTCCCAAGCACTCAGGTACTTATATCTGCGATGGGGAATG2469                           AlaSerArgSerGlnAlaLeuArgTyrLeuTyrLeuArgTrpGlyMet                               770775780                                                                      GTCCCTGTACTGGCATCTCGGTCCCAAGCACTCAGGTACTTATATCTG2517                           ValProValLeuAlaSerArgSerGlnAlaLeuArgTyrLeuTyrLeu                               785790795800                                                                   CGATGGGGAATGGTCCCTGTACTGGCATCTCGGTCCCAAGCACTCAGG2565                           ArgTrpGlyMetValProValLeuAlaSerArgSerGlnAlaLeuArg                               805810815                                                                      TACTTATATCTGCGATGGGGAATGGACTTGTCGAAGTTGGTGGTTTTC2613                           TyrLeuTyrLeuArgTrpGlyMetAspLeuSerLysLeuValValPhe                               820825830                                                                      GTCGGAGAAAGTGGTGATACCGATTATGAAGGATTGATCGGTGGTCTA2661                           ValGlyGluSerGlyAspThrAspTyrGluGlyLeuIleGlyGlyLeu                               835840845                                                                      CGCAAGGCTGTCATAATGAAAGGACTCTGCACTAATGCAAGCAGCTTA2709                           ArgLysAlaValIleMetLysGlyLeuCysThrAsnAlaSerSerLeu                               850855860                                                                      ATTCACGGTAATAGGAATTACCCGCTATCTGATGTTTTACCATTCGAG2757                           IleHisGlyAsnArgAsnTyrProLeuSerAspValLeuProPheGlu                               865870875880                                                                   AGCCCTAATGTCATCCAAGCGGATGAGGAATGTAGCAGCACCGGAATC2805                           SerProAsnValIleGlnAlaAspGluGluCysSerSerThrGlyIle                               885890895                                                                      CGTTCCTTACTGGAGAAACTAGCGGTACTCAAAGGATAATACCCTT2851                             ArgSerLeuLeuGluLysLeuAlaValLeuLysGly                                           900905                                                                         CCCCCTTTGATTGTCAAAAACCTATATGAGCTAAGATTATGCCATGAAAAGAATGGCCAT2911               CCATTTGGCTTGTCTTTTG2930                                                        (2) INFORMATION FOR SEQ ID NO:6:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 908 amino acids                                                    (B) TYPE: amino acid                                                           (D) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: protein                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:                                        MetAlaGlyAsnAspTrpIleAsnSerTyrLeuGluAlaIleLeuAsp                               151015                                                                         ValGlyProGlyLeuAspAspLysLysSerSerLeuLeuLeuArgGlu                               202530                                                                         ArgGlyArgPheSerProThrArgTyrPheValGluGluValIleThr                               354045                                                                         GlyPheAspGluThrAspLeuHisArgSerTrpIleArgAlaGlnAla                               505560                                                                         ThrArgSerProGlnGluArgAsnThrArgLeuGluAsnMetCysTrp                               65707580                                                                       ArgIleTrpAsnLeuAlaArgGlnLysLysGlnLeuGluGlyGluGln                               859095                                                                         AlaGlnTrpMetAlaLysArgArgGlnGluArgGluArgGlyArgArg                               100105110                                                                      GluAlaValAlaAspMetSerGluAspLeuSerGluGlyGluLysGly                               115120125                                                                      AspIleValAlaAspMetSerSerHisGlyGluSerThrArgGlyArg                               130135140                                                                      LeuProArgIleSerSerValGluThrMetGluAlaTrpValSerGln                               145150155160                                                                   GlnArgGlyLysLysLeuTyrIleValLeuIleSerLeuHisGlyLeu                               165170175                                                                      IleArgGlyGluAsnMetGluLeuGlyArgAspSerAspThrGlyGly                               180185190                                                                      GlnValLysTyrValValGlyAlaThrValAlaGlnGlyArgLeuSer                               195200205                                                                      LysAspGluIleAsnSerThrTyrLysIleMetArgArgIleGluAla                               210215220                                                                      GluGluLeuThrLeuAspAlaSerGluIleValIleThrSerThrArg                               225230235240                                                                   GlnGluIleAspGluGlnTrpArgLeuTyrAspGlyPheAspProIle                               245250255                                                                      LeuGluArgLysLeuArgAlaArgIleLysArgAsnValSerCysTyr                               260265270                                                                      GlyArgPheMetProArgMetAlaValIleProProGlyMetGluPhe                               275280285                                                                      HisHisIleValProHisGluGlyAspMetAspGlyGluThrGluGly                               290295300                                                                      SerGluAspGlyLysThrProAspProProIleTrpAlaGluIleMet                               305310315320                                                                   ArgPhePheSerAsnProArgLysProMetIleLeuAlaLeuAlaArg                               325330335                                                                      ProAspProLysLysAsnLeuThrThrLeuValLysAlaPheGlyGlu                               340345350                                                                      CysArgProLeuArgAspLeuAlaAsnLeuThrLeuIleMetGlyAsn                               355360365                                                                      ArgAspAsnIleAspGluMetSerSerThrAsnSerAlaLeuLeuLeu                               370375380                                                                      SerIleLeuLysMetIleAspLysTyrAspLeuTyrGlyLeuValAla                               385390395400                                                                   TyrProLysHisHisLysGlnSerAspValProAspIleTyrArgLeu                               405410415                                                                      AlaAlaLysThrLysGlyValPheIleAsnProAlaPheIleGluPro                               420425430                                                                      PheGlyLeuThrLeuIleGluAlaAlaAlaTyrGlyLeuProMetVal                               435440445                                                                      AlaThrLysAsnGlyGlyProValAspIleHisArgValLeuAspAsn                               450455460                                                                      GlyLeuLeuValAspProHisAspGlnGlnAlaIleAlaAspAlaLeu                               465470475480                                                                   LeuLysLeuValAlaAspLysGlnLeuTrpAlaLysCysArgAlaAsn                               485490495                                                                      GlyLeuLysAsnIleHisLeuPheSerTrpProGluHisCysLysThr                               500505510                                                                      TyrLeuSerArgIleAlaSerCysLysProArgGlnHisSerLeuArg                               515520525                                                                      AspIleHisAspIleSerLeuAsnLeuArgPheSerLeuAspGlyGlu                               530535540                                                                      LysAsnAspAsnLysGluAsnAlaAspAsnThrLeuAspProGluVal                               545550555560                                                                   ArgArgSerLysLeuGluAsnAlaValLeuSerLeuSerLysGlyAla                               565570575                                                                      LeuLysSerThrSerLysSerTrpSerSerAspLysAlaAspGlnAsn                               580585590                                                                      ProGlyAlaGlyLysPheProAlaIleArgArgArgArgHisIlePhe                               595600605                                                                      ValIleAlaValAspCysAspAlaSerSerGlyLeuSerGlySerMet                               610615620                                                                      LysLysIlePheGluAlaValGluLysGluArgAlaGluGlySerIle                               625630635640                                                                   GlyPheIleLeuAlaThrSerPheAsnIleSerGluValGlnSerPhe                               645650655                                                                      LeuLeuSerGluGlyMetAsnProThrGluGlnAsnProPheValVal                               660665670                                                                      AspLeuTyrTyrHisSerHisIleGluTyrArgTrpGlyGlyGluGly                               675680685                                                                      LeuArgLysThrLeuValArgTrpAlaAlaSerIleIleAspLysAsn                               690695700                                                                      GlyGluAsnGlyAspHisIleValValGluAspGluAspAsnSerAla                               705710715720                                                                   AspTyrCysTyrThrPheLysValCysLysProGlyThrValProPro                               725730735                                                                      SerLysGluLeuArgLysValMetArgIleGlnAlaLeuArgCysHis                               740745750                                                                      AlaValTyrCysGlnAsnGlySerArgIleAsnValIleProValLeu                               755760765                                                                      AlaSerArgSerGlnAlaLeuArgTyrLeuTyrLeuArgTrpGlyMet                               770775780                                                                      ValProValLeuAlaSerArgSerGlnAlaLeuArgTyrLeuTyrLeu                               785790795800                                                                   ArgTrpGlyMetValProValLeuAlaSerArgSerGlnAlaLeuArg                               805810815                                                                      TyrLeuTyrLeuArgTrpGlyMetAspLeuSerLysLeuValValPhe                               820825830                                                                      ValGlyGluSerGlyAspThrAspTyrGluGlyLeuIleGlyGlyLeu                               835840845                                                                      ArgLysAlaValIleMetLysGlyLeuCysThrAsnAlaSerSerLeu                               850855860                                                                      IleHisGlyAsnArgAsnTyrProLeuSerAspValLeuProPheGlu                               865870875880                                                                   SerProAsnValIleGlnAlaAspGluGluCysSerSerThrGlyIle                               885890895                                                                      ArgSerLeuLeuGluLysLeuAlaValLeuLysGly                                           900905                                                                         __________________________________________________________________________ 

I claim:
 1. A transgenic plant with an altered level of sucrose-phosphate-synthase activity relative to a nontransformed plant, wherein a chimeric DNA construct comprising a DNA fragment selected from the group consisting of the coding region of SEQ ID No. 1, the coding region of SEQ ID No. 3 and the coding region of SEQ ID No. 5 has been transformed into said plant.
 2. A transgenic plant, as recited in claim 1, wherein said plant is a potato plant.
 3. A transgenic plant with an altered level of sucrose-phosphate-synthase activity relative to a nontransformed plant, wherein a chimeric DNA construct comprising a DNA fragment encoding sucrose-phosphate-synthase which comprises an amino acid sequence selected from the group consisting of SEQ ID No. 2, SEQ ID No. 4 and SEQ ID No. 6 has been transformed into said plant.
 4. A DNA fragment comprising the coding region of SEQ ID No. 1 which begins at nucleotide posistion
 957. 5. A DNA fragment comprising the coding region of SEQ ID No. 3 which begins at nucleotide posistion
 121. 6. A DNA fragment comprising the coding region of SEQ ID No. 5 which begins at nucleotide posistion
 118. 7. A plasmid, comprising:a) a promoter sequence; b) at least one DNA fragment selected from the group consisting of the coding region of SEQ ID No. 1, the coding region of SEQ ID No. 3 and the coding region of SEQ ID No. 5, wherein said DNA fragment is operably linked to said promoter; and c) a non-coding termination sequence operably linked to said DNA fragment.
 8. A plasmid, comprising:a) a promoter sequence; b) at least one DNA fragment encoding sucrose-phosphate-synthase wherein said sucrose-phosphate-synthase comprises an amino acid sequence selected from the group consisting of SEQ ID No. 2, SEQ ID No. 4 and SEQ ID No. 6, wherein said DNA fragment is operably linked to said promoter; and c) a non-coding termination sequence operably linked to said DNA fragment.
 9. A method of producing a plant with an altered level of sucrose-phosphate-synthase activity relative to a nontransformed plant, comprising the step of:overexpressing a DNA sequence according to claim 4 in a plant.
 10. A method of producing a plant with an altered level of sucrose-phosphate-synthase activity relative to a nontransformed plant, comprising the step of:overexpressing a DNA sequence according to claim 5 in a plant.
 11. A method of producing a plant with an altered level of sucrose-phosphate-synthase activity relative to a nontransformed plant, comprising the step of:overexpressing a DNA sequence according to claim 6 in a plant.
 12. A method of producing a plant with an altered level of sucrose-phosphate-synthase activity relative to a nontransformed plant, comprising the steps of:transforming a plant cell with a plasmid according to claim 7; and regenerating a plant from said transformed plant cell.
 13. A method of producing a plant with an altered level of sucrose-phosphate-synthase activity relative to a nontransformed plant, comprising the steps of:transforming a plant cell with a plasmid according to claim 8; and regenerating a plant from said transformed plant cell.
 14. A DNA fragment encoding a sucrose-phosphate-synthase protein wherein said sucrose-phosphate-synthase comprises an amino acid sequence as shown in SEQ ID No.
 2. 15. A DNA fragment encoding a sucrose-phosphate-synthase protein wherein said sucrose-phosphate-synthase comprises an amino acid sequence as shown in SEQ ID No.
 4. 16. A DNA fragment encoding a sucrose-phosphate-synthase protein wherein said sucrose-phosphate-synthase comprises an amino acid sequence as shown in SEQ ID No.
 6. 